Mesenchymal stem cell transplantation in a nitrofen-induced congenital diaphragmatic hernia rat model

本研究的目的是评价间充质干细胞在硝芬诱导的CDH大鼠模型中的作用。怀孕大鼠在E9.5暴露于硝芬。从eGFP转基因大鼠肺中分离出间充质干细胞。将MSCs经子宫静脉移植到硝芬诱导的E12.5大鼠体内，收获E21肺外植体。实验动物分为三组：对照组、CDH组和msc处理的CDH组。采用HE和免疫组化染色对标本进行形态学分析。骨髓间充质干细胞处理的CDH组肺动脉肺泡壁和内侧壁较CDH组明显变薄。肺泡间隙面积明显大于CDH组。骨髓间充质干细胞移植可能促进肺泡和肺动脉的发育，从而减轻肺发育不全的严重程度。

The aim of this study was to evaluate the efficacy of MSCs in a nitrofen-induced CDH rat model.Pregnant rats were exposed to nitrofen on E9.5. MSCs were isolated from the eGFP transgenic rat lungs. The MSCs were transplanted into the nitrofen-induced E12.5 rats via the uterine vein, and the E21 lung explants were harvested. The study animals were divided into three: the control group, the CDH group, and the MSC-treated CDH group. The specimens were morphologically analyzed using HE and immunohistochemical staining. The alveolar and medial walls of the pulmonary arteries were significantly thinner in the MSC-treated CDH group than in the CDH group. The alveolar air space areas were larger than in the CDH group. MSC transplantation potentially promotes alveolar and pulmonary artery development, thereby reducing the severity of pulmonary hypoplasia.