Mesenchymal stem cell transplantation in a nitrofen-induced congenital diaphragmatic hernia rat model

本研究的目的是评估骨髓间充质干细胞在除草醚诱导的CDH大鼠模型中的疗效。从eGFP转基因大鼠肺中分离MSC。将MSCs经子宫静脉移植到硝苯草醚诱导的E12. 5大鼠体内，并收集E21肺组织块。实验动物分为对照组、CDH组和MSC治疗CDH组。采用HE和免疫组织化学染色对标本进行形态学分析。与CDH组相比，MSC治疗的CDH组的肺动脉肺泡壁和中膜壁明显变薄。肺泡气腔面积大于CDH组。MSC移植可能促进肺泡和肺动脉发育，从而降低肺发育不良的严重程度。

The aim of this study was to evaluate the efficacy of MSCs in a nitrofen-induced CDH rat model.Pregnant rats were exposed to nitrofen on E9.5. MSCs were isolated from the eGFP transgenic rat lungs. The MSCs were transplanted into the nitrofen-induced E12.5 rats via the uterine vein, and the E21 lung explants were harvested. The study animals were divided into three: the control group, the CDH group, and the MSC-treated CDH group. The specimens were morphologically analyzed using HE and immunohistochemical staining. The alveolar and medial walls of the pulmonary arteries were significantly thinner in the MSC-treated CDH group than in the CDH group. The alveolar air space areas were larger than in the CDH group. MSC transplantation potentially promotes alveolar and pulmonary artery development, thereby reducing the severity of pulmonary hypoplasia.