Mesenchymal stem cell transplantation in a nitrofen-induced congenital diaphragmatic hernia rat model

间充质干细胞移植治疗除草醚诱导的先天性膈疝大鼠模型

• 批准号: 23792031
• 负责人: NAGATA Kouji
• 金额: $ 2.66万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792031/
• 关键词: 再生医療; 細胞治療; 間葉系幹細胞; 肺低形成; 肺発達; 先天性横隔膜ヘルニア; 骨髄幹細胞移植; 肺発生; 再生

The aim of this study was to evaluate the efficacy of MSCs in a nitrofen-induced CDH rat model.Pregnant rats were exposed to nitrofen on E9.5. MSCs were isolated from the eGFP transgenic rat lungs. The MSCs were transplanted into the nitrofen-induced E12.5 rats via the uterine vein, and the E21 lung explants were harvested. The study animals were divided into three: the control group, the CDH group, and the MSC-treated CDH group. The specimens were morphologically analyzed using HE and immunohistochemical staining. The alveolar and medial walls of the pulmonary arteries were significantly thinner in the MSC-treated CDH group than in the CDH group. The alveolar air space areas were larger than in the CDH group. MSC transplantation potentially promotes alveolar and pulmonary artery development, thereby reducing the severity of pulmonary hypoplasia.

本研究的目的是评价骨髓间充质干细胞在Nitfen诱导的CDH大鼠模型中的效果。从绿色荧光蛋白转基因大鼠肺中分离出骨髓间充质干细胞。将骨髓间充质干细胞经子宫静脉移植到硝苯醚诱导的E12.5大鼠体内，取E21肺组织块。实验动物分为三组：对照组、CDH组和MSC处理CDH组。采用HE染色和免疫组织化学染色对标本进行形态分析。经MSC处理的CDH组的肺泡壁和内侧壁较CDH组明显变薄。肺泡腔面积明显大于CDH组。骨髓间充质干细胞移植有可能促进肺泡和肺动脉发育，从而减轻肺发育不良的严重程度。

项目成果

Therapeutic potential of mesenchymal stem cell transplantation in a nitrofen-induced congenital diaphragmatic hernia rat model

• DOI: 10.1007/s00383-014-3576-9
• 发表时间: 2014-08
• 期刊: Pediatric Surgery International
• 影响因子: 1.8
• 作者: Ratih Yuniartha;F. Alatas;Kouji Nagata;Masaaki Kuda;Y. Yanagi;Genshiro Esumi;T. Yamaza;Y. Kinoshita;T. Taguchi

腸管壁内気腫を呈した短腸症候群の1例

短肠综合征合并肠气肿1例

• 发表时间: 2013
• 作者: 永田公二;江角元史郎;増本幸二;田口智章
• 通讯作者: 田口智章

The current profile and the future perspectives of congenital diaphragmatic hernia – A nationwide survey in Japan –

先天性膈疝的现状和未来展望 – 日本全国调查 –

• 发表时间: 2013
• 作者: Nagata K,Usui N;Kanamori Y;Takahashi S;Hayakawa M; Okuyama H;Inamura N,Taguchi T
• 通讯作者: Inamura N,Taguchi T

小児在宅静脈栄養患者に対する多職種連携の取り組み

儿科家庭肠外营养患者的多学科协作努力

当科における超短腸症候群の栄養管理の実際/腸管機能不全長期生存例の問題点

我科极短肠综合征的实用营养管理/肠功能障碍长期生存病例的问题