Analysis of supporting abilities for undifferentiated dental pulp stem cells by their microenvironments

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The expressions of mRNA and cytokines of PDGFRalpha and Sca-1 double positive dental pulp stem cells (DPSCs) were compared to bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs). These mRNA and cytokines expressions did not show significant difference between DPSCs and BM-MSCs except Cxcl12 mRNA expression. We investigated whether proliferation and differentiation potentials of passaged DPSCs are improved by hypoxic culture. The DPSCs well maintained a proliferation and differentiation potentials with passage under the hypoxic culture condition. These results suggest that the proliferation and differentiation potentials of DPSCs were maintained in hypoxic condition. Then, hematopoietic stem/progenitor cells (HSPCs) supporting activity of DPSCs was analyzed by in vitro co-culture system. Moreover, their HSC-supporting activity was evaluated by in vivo xenotransplantation assays using NOG mice. The DPSCs as well as BM-MSCs supported human cord blood-derived HSCs.