Analysis of supporting abilities for undifferentiated dental pulp stem cells by their microenvironments

微环境对未分化牙髓干细胞的支持能力分析

• 批准号: 23792304
• 负责人: NAKATSUKA Ryusuke
• 金额: $ 2.66万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23792304/
• 关键词: 歯髄幹細胞; 細胞外環境; 低酸素; 造血細胞支持; E-cadherin; 上皮細胞; 間葉細胞; 造血制御; 再生歯学

The expressions of mRNA and cytokines of PDGFRalpha and Sca-1 double positive dental pulp stem cells (DPSCs) were compared to bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs). These mRNA and cytokines expressions did not show significant difference between DPSCs and BM-MSCs except Cxcl12 mRNA expression. We investigated whether proliferation and differentiation potentials of passaged DPSCs are improved by hypoxic culture. The DPSCs well maintained a proliferation and differentiation potentials with passage under the hypoxic culture condition. These results suggest that the proliferation and differentiation potentials of DPSCs were maintained in hypoxic condition. Then, hematopoietic stem/progenitor cells (HSPCs) supporting activity of DPSCs was analyzed by in vitro co-culture system. Moreover, their HSC-supporting activity was evaluated by in vivo xenotransplantation assays using NOG mice. The DPSCs as well as BM-MSCs supported human cord blood-derived HSCs.

将 PDGFRα 和 Sca-1 双阳性牙髓干细胞 (DPSC) 的 mRNA 和细胞因子的表达与骨髓源性间充质干/基质细胞 (BM-MSC) 进行比较。除Cxcl12 mRNA表达外，DPSCs和BM-MSCs之间这些mRNA和细胞因子表达没有显示出显着差异。我们研究了低氧培养是否可以提高传代 DPSC 的增殖和分化潜力。 DPSCs在低氧培养条件下传代仍保持良好的增殖和分化潜能。这些结果表明DPSCs的增殖和分化潜力在低氧条件下得以维持。然后，通过体外共培养系统分析支持 DPSC 活性的造血干/祖细胞 (HSPC)。此外，使用 NOG 小鼠进行体内异种移植试验评估了它们的 HSC 支持活性。 DPSC 以及 BM-MSC 支持人脐带血来源的 HSC。

项目成果

Development of a higly efficient method for isolating bone-derived small stem cells adentified in adult mouse bone

开发一种高效方法来分离成年小鼠骨骼中鉴定出的骨源性小干细胞

• 发表时间: 2013
• 作者: 中塚 隆介;岩城 隆二;松岡 由和;高橋 雅也;藤岡 龍哉;佐々木 豊;薗田 精昭
• 通讯作者: 薗田 精昭

Hypoxic culture condition facilitates the proliferation and differentiation potentials of mouse dental pulp stem cells

低氧培养条件有利于小鼠牙髓干细胞的增殖和分化潜能

• 发表时间: 2011
• 作者: Ryusuke Nakatsuka;他
• 通讯作者: 他

Mouse Dental Pulp Stem Cells Support Human Umbilical Cord Blood-Derived Hematopoietic Stem/Progenitor Cells in Vitro

• DOI: 10.3727/096368913x674675
• 发表时间: 2015-01
• 期刊: Cell Transplantation
• 影响因子: 3.3
• 作者: Ryusuke Nakatsuka;Yoshikazu Matsuoka;Y. Uemura;Keisuke Sumide;Ryuji Iwaki;Masaya Takahashi;T. Fujioka;Yutaka Sasaki;Y. Sonoda

マウス骨質由来微小幹細胞の同定・分離法の開発とその幹細胞特性の解明

小鼠骨源性微干细胞鉴定分离方法的建立及其干细胞特性的阐明

• 发表时间: 2013
• 作者: 岩城隆二;中塚隆介;他
• 通讯作者: 他

Development of a higly efficient method for isolating bone-derived small stem cells identified in adult mouse bone

开发一种高效方法来分离成年小鼠骨骼中鉴定的骨源性小干细胞

• 发表时间: 2013
• 作者: 中塚隆介;他
• 通讯作者: 他