An investigation of tissue regeneration therapy using dental pulp-derived CD45(-), Sca-1(+) and CXCR4(+) cells transplantation.

在这项研究中，我们研究了这些DPSC是否可以通过流式细胞仪使用骨髓来源的间充质干细胞（MSC）标记物进行前瞻性分离。此外，这些DPSC的特性已被广泛研究，使用流式细胞仪，免疫染色，并在体外组织定向分化培养。本研究开发了一种新的方法来前瞻性地分离牙髓来源的小鼠DPSCs，并揭示了所鉴定的DPSCs具有分化成类似于MSC的多个谱系的潜力，并且这些细胞存在于牙齿形成细胞的龛中。

In this study, we investigated whether these DPSCs can be prospectively isolated by FACS using bone marrow-derived mesenchymal stem cell (MSC) markers. Furthermore, characteristics of these DPSCs have been extensively investigated using FACS, immunostaining, and in vitro tissue-orientetd differentiation cultures. This study developed a novel method to prospectively isolate the dental pulp-derived mouse DPSCs and revealed that the identified DPSCs possess the potential to differentiate into multiple lineages similar to the MSCs, and these cells exist in the niche for tooth forming cells.