Detecting tumor biomarker using aptamer-based highly-sensitive potentiometric biosensor

使用基于适配体的高灵敏电位生物传感器检测肿瘤生物标志物

• 批准号: 23750079
• 负责人: GODA Tatsuro
• 金额: $ 2.91万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23750079/
• 关键词: アプタマー; バイオマーカー; タンパク質; 電界効果トランジスタ; バイオセンサー; 電位計測; 自己組織化単分子膜; タンパク質バイオマーカー; 腫瘍マーカー; ラベルフリー検出; ラベルフリー技術

A highly sensitive detection of serum protein as a biomarker for cancer and/or tumor was conducted by using aptamer-based field-effect transistor (FET) biosensor without the need for labeling. The aptamer immobilized on the semiconductor-based biosensor specifically captured target protein within the electrical double layer which is spontaneously formed at the electrolyte/sensor interface. The FET sensor successfully detected the innate net-charge of the captured protein by the field effect. The technology allows a fast and low-cost detection of the biomarkers in a massively parallel format, providing a new platform for electrical sensing of proteins, proteomics, and post-translational modifications as an alternative way to conventional immuno-assays coupled with optical readout. The fundamental knowledge obtained by the project can be applied for clinical diagnosis for cancers, leading an innovation for current medical system.

使用基于适配体的场效应晶体管（FET）生物传感器对作为癌症和/或肿瘤生物标志物的血清蛋白进行高灵敏度检测，无需标记。固定在基于半导体的生物传感器上的适体特异性地捕获在电解质/传感器界面处自发形成的双电层内的目标蛋白质。 FET 传感器通过场效应成功检测到捕获蛋白质的固有净电荷。该技术允许以大规模并行格式快速、低成本地检测生物标记物，为蛋白质、蛋白质组学和翻译后修饰的电传感提供新的平台，作为与光学读数相结合的传统免疫测定的替代方法。该项目获得的基础知识可应用于癌症的临床诊断，引领当前医疗体系的创新。

项目成果

Label-free and reagent-less protein biosensing using aptamer-modified extended-gate field-effect transistors

• DOI: 10.1016/j.bios.2013.01.053
• 发表时间: 2013-07-15
• 期刊: BIOSENSORS & BIOELECTRONICS
• 影响因子: 12.6
• 作者: Goda, Tatsuro;Miyahara, Yuji
• 通讯作者: Miyahara, Yuji

A hairpin DNA aptamer coupled with groove binders as a smart switch for a field-effect transistor biosensor

• DOI: 10.1016/j.bios.2011.12.022
• 发表时间: 2012-02-15
• 期刊: BIOSENSORS & BIOELECTRONICS
• 影响因子: 12.6
• 作者: Goda, Tatsuro;Miyahara, Yuji
• 通讯作者: Miyahara, Yuji

Detection of Biomolecular Recognition Using Biotransistors

使用生物晶体管进行生物分子识别检测

• 发表时间: 2012
• 作者: Yuji Miyahara;Akira Matsumoto;Tatsuro Goda;Yasuhiro Maeda;Miyuki Tabata;Mai Sanjoh
• 通讯作者: Mai Sanjoh

Interpretation of Protein Adsorption through Its Intrinsic Electric Charges: A Comparative Study Using a Field-Effect Transistor, Surface Plasmon Resonance, and Quartz Crystal Microbalance

• DOI: 10.1021/la302977s
• 发表时间: 2012-10-16
• 期刊: LANGMUIR
• 影响因子: 3.9
• 作者: Goda, Tatsuro;Miyahara, Yuji
• 通讯作者: Miyahara, Yuji

ヘアピン型DNAアプタマーのナノ構造変化を利用したATP検出

利用发夹状 DNA 适体纳米结构变化检测 ATP

• 发表时间: 2011
• 作者: 合田達郎;宮原裕二
• 通讯作者: 宮原裕二