Detecting tumor biomarker using aptamer-based highly-sensitive potentiometric biosensor

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A highly sensitive detection of serum protein as a biomarker for cancer and/or tumor was conducted by using aptamer-based field-effect transistor (FET) biosensor without the need for labeling. The aptamer immobilized on the semiconductor-based biosensor specifically captured target protein within the electrical double layer which is spontaneously formed at the electrolyte/sensor interface. The FET sensor successfully detected the innate net-charge of the captured protein by the field effect. The technology allows a fast and low-cost detection of the biomarkers in a massively parallel format, providing a new platform for electrical sensing of proteins, proteomics, and post-translational modifications as an alternative way to conventional immuno-assays coupled with optical readout. The fundamental knowledge obtained by the project can be applied for clinical diagnosis for cancers, leading an innovation for current medical system.