Real-time imaging of excitation-secretioncoupling process from the single peptidergic neuron

为了解多肽能神经元对中枢神经系统的微调机制，本研究利用离体鱼末梢神经(TN)-促性腺激素释放激素（GnRH）神经元的原代培养系统，研究了单个多肽能神经元的兴奋-分泌关系以及单细胞电穿孔外源基因的基因转移。因此，我们能够测量单个肽能神经元亚细胞区域的分泌囊泡运动及其胞外活动。我们还分析了作为GnRH肽分泌来源的n -GnRH神经元的放电模式控制的离子机制。

To understand fine tuning mechanisms of the central nervous system by peptidergic neurons, we investigated the excitation-secretion relationship of single peptidergic neuron using a primary culture system of isolated fish terminal nerve (TN)-gonadotropin releasing hormone (GnRH) neuron and gene transfer of exogenous genes by single cell electroporation. As a result, we enabled measurement of the movement of secretory vesicles and their exocytotic events from the subcellular region of single peptidergic neuron. We also analyzed the ionic mechanisms of the firing pattern control of TN-GnRH neurons that are the origin of exocytosis of GnRH peptide.