Role of RBM42 in proliferation and survival

RBM42 在增殖和存活中的作用

• 批准号: 23770199
• 负责人: NAIKI Takahiro
• 金额: $ 2.91万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770199/
• 关键词: RNA結合タンパク質; 細胞周期

Post-transcriptional regulation of gene expression by RNA-binding proteins has pivotal roles in many biological processes. We analyzed role of two RNA-binding proteins, RBM42 and hnRNPK, in cell proliferation. In RBM42 and hnRNPK knockdown cells, G1/S progression was delayed as compared to control cells. E2F is an essential transcription factor that is required for gene expression in G1/S transition. The expression of E2F target genes was significantly reduced in RBM42 and hnRNPK knockdown cells. Furthermore, we found that hnRNPK associates with E2F mRNA and regulates the mRNA stability of E2F mRNA. These results suggest that hnRNPK positively regulates cell cycle progression through the regulation of E2F mRNA stability.

通过RNA结合蛋白对基因表达的转录后调节在许多生物过程中具有关键作用。我们分析了两种RNA结合蛋白RBM42和HNRNPK在细胞增殖中的作用。在RBM42和HNRNPK敲低细胞中，与对照细胞相比，G1/S的进展被延迟。 E2F是G1/s转变中基因表达所需的必不可少的转录因子。在RBM42和HNRNPK敲除细胞中，E2F靶基因的表达显着降低。此外，我们发现HNRNPK与E2F mRNA相关并调节E2F mRNA的mRNA稳定性。这些结果表明，HNRNPK通过调节E2F mRNA稳定性来积极调节细胞周期的进程。

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Stau1 regulates Dvl2 expressionduring myoblast differentiation

Stau1 在成肌细胞分化过程中调节 Dvl2 表达

• 发表时间: 2012
• 期刊: Biochem Biophys Res Commun
• 作者: Yamaguchi Y;Naiki T;Irie K
• 通讯作者: Irie K