Downregulation o fserine/arginine-rich splicing factor 3 induces G1 cell cycle arrest and apoptosis in colon cancer cells
Downregulation o fserine/arginine-rich splicing factor 3 induces G1 cell cycle arrest and apoptosis in colon cancer cells
批准号:
23590943
负责人:
TANAHASHI Toshihito
金额:
$3.58万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2013
中文摘要
富丝氨酸/精氨酸剪接因子3 (SRSF3)是SRSF家族的一员,在基因表达中起着广泛的作用。SRSF3敲低诱导结肠癌细胞G1阻滞和凋亡(HCT116)。对SRSF3沉默细胞和过表达SRSF3免疫沉淀mrna中差异表达转录物的通路分析表明,SRSF3优先与G1/S检查点调节mrna相关并调节其表达。SRSF3敲除降低细胞周期蛋白(D1、D3和E1)、E2F1和E2F7的mRNA和蛋白水平。SRSF3敲除导致hipk2外显子8的81个核苷酸跳跃。由此产生的HIPK2异构体部分缺乏E3泛素连接酶结合位点,对蛋白酶体消化产生抗性。在多种表达野生型或突变型p53的结肠癌细胞中,通过选择性分解全长hipk2 mRNA和敲低SERF3诱导凋亡的剪接变异体的主要表达。
英文摘要
Serine/arginine-rich splicing factor 3 (SRSF3) is a member of the SRSF family that plays wide-ranging roles in gene expression. SRSF3 knockdown induced G1 arrest and apoptosis in colon cancer cells (HCT116). Pathway analysis of differentially expressed transcripts in SRSF3-silenced cells and mRNAs immunoprecipitated with overexpressed SRSF3 suggested that SRSF3 preferentially associated with G1/S checkpoint regulator mRNAs and regulated their expression. SRSF3 knockdown decreased mRNA and protein levels of cyclins (D1, D3, and E1), E2F1, and E2F7. SRSF3 knockdown caused skipping of 81 nucleotides of the hipk2 exon 8. Resultant HIPK2 isoform partially lacking the E3 ubiquitin ligase-binding site became resistant to proteasome digestion. Predominant expression of the splice variant by selective decomposition of full-length hipk2 mRNA as well as by SERF3 knockdown induced apoptosis in a variety of colon cancer cells expressing wild-type or mutated p53.
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Hu antigen R (huR) functions as an alternative pre-mRNA splicing enhancer of transformer 2-beta (Tra2beta) on exon definition under oxidative stress
Hu 抗原 R (huR) 在氧化应激下作为 Transformer 2-beta (Tra2beta) 外显子定义的替代前 mRNA 剪接增强子
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Masuda K, Yamagishi N, Kurokawa K, Satake Y, Kajita K, Akaike Y, Honda M, Nishida K, Kuwano Y, Tanahashi T, Rokutan K.]
通讯作者:
Rokutan K.
Premature stop codon-containing tra2beta4 mRNA isoform regulates cellular senescence
含有过早终止密码子的 tra2beta4 mRNA 亚型调节细胞衰老
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Kajita K, Kuwano Y, Masuda K, Yamagishi N, Kurokawa K, Satake Y, Akaike Y, Honda M, Tanahashi T, Rokutan K]
通讯作者:
Rokutan K
Serine/arginine-rich splicing factor 3 (SRSF3) regulates G1/S checkpoint and p53-dependent apoptosis
富含丝氨酸/精氨酸的剪接因子 3 (SRSF3) 调节 G1/S 检查点和 p53 依赖性细胞凋亡
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Kurokawa K, Akaike Y, Kajita K, Yamagishi N, Satake Y, Honda M, Nishida K, Kuwano Y, Masuda K, Tanahashi T, Rokutan K]
通讯作者:
Rokutan K
酸化ストレスと選択的スプライシングのクロストーク
氧化应激和选择性剪接之间的串扰
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[棚橋俊仁, 黒川憲, 六反一仁]
通讯作者:
六反一仁
Transfomer-2beta regulates apoptosis through post-transcriptional regulation of bcl-2
Transfomer-2beta 通过 bcl-2 转录后调节调节细胞凋亡
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Kuwano Y, Kajita K, Satake Y, Kurokawa K, Yamagishi N, Akaike Y, Honda M, Nishida K, Masuda K, Tanahashi T, Rokutan K]
通讯作者:
Rokutan K
共 8 条
Whole-genome sequencing of clarithromycin resistant Helicobacter pylori characterizes unidentified variants of multidrug resistant efflux pump genes
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批准号:26460212
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$3.16万
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财政年份:2014
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负责人:TANAHASHI Toshihito
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依托单位:
海外基金