Characterization of Replication fork DNA specific motor proteins using single molecule analysis

单分子成像技术使我们能够测量和调节蛋白质或DNA的运动。利用这些技术，我确定了E。coliUvrD解旋酶和RuvB马达蛋白，它们促进Holliday连接DNA的分支迁移。我还测量了吡咯-咪唑聚酰胺的缔合和解离速率，并计算了它们的模型结构，以阐明DNA结合动力学与吡咯-咪唑聚酰胺结构之间的关系。

Single molecule imaging techniques enable us to measure and regulate movements of proteins or DNA. Using these techniques, I characterized E. coli UvrD helicase and RuvB motor protein, which promote branch migration of Holliday junction DNA. I also measured association and dissociation rates of Pyrrole-Imidazole polyamides and calculated their model structures to clarify the relationship between the DNA binding kinetics and Pyrrole-Imidazole polyamide structures.