Elucidation of the selective lignin degradation mechanism in a white rot fungus and the biosynthetic pathway of its key metabolites through a proteomic approach

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A white-rot fungus, Ceriporiospsis subvermispora, can selectively degrade lignin without serious damage to cellulose. In order to elucidate the molecular mechanism of the selective lignin degradation, we tried to investigate the cellular response of C. subvermispora to a lignin fragment, vanillin. We obtained expression profiles of C. subvermispora in the presence and absence of vanillin through a proteomics-based approach, and detected vanillin-induced and repressed proteins. Using suppression subtractive hybridization, moreover, we also obtained many genes clones that are up-regulated in the presence of vanillin.