Screening of anti-virus activities from deep-sea-adapted bacteria

筛选适应深海的细菌的抗病毒活性

• 批准号: 23780198
• 负责人: KAWANO Hiroaki
• 金额: $ 2.91万
• 依托单位: Osaka University of Pharmaceutical Sciences (2012)Nagasaki University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23780198/
• 关键词: 微生物; 深海; 深海微生物; 多様性; グラム陽性菌; 抗ウイルス活性; 核外輸送阻害剤

We isolated deep-sea-adapted bacteria from deep-sea-mud samples collected at the Japan sea, Sagami bay, and Japan trench. These bacteria were stored as a bacterial library and their diversity was analyzed based on the 16S rDNA nucleotide sequences. One thousand bacteria were cultured and the resulting supernatants were stored so as to be used for anti-virus activity screening. In addition, establishment of EGFP/NLS/NES cell line which will be used for anti-virus activity screening was successfully achieved. Furthermore, we found significant anti-influenza virus activities in13 supernatant mixtures of deep-sea bacterial cultures.

我们从日本海、相模湾和日本海沟采集的深海泥样本中分离出适应深海的细菌。将这些细菌储存为细菌库，并根据16S rDNA核苷酸序列分析它们的多样性。培养了1000个细菌，并将所得上清液储存起来，用于抗病毒活性的筛选。此外，成功建立了用于抗病毒活性筛选的EGFP/NLS/NES细胞系。此外，我们在13种深海细菌培养上清液中发现了显著的抗流感病毒活性。

项目成果

A new cell-based reporter system for sensitive screening of nuclear export inhibitors

• DOI: 10.5582/ddt.2011.v5.6.286
• 发表时间: 2011-01-01
• 期刊: DRUG DISCOVERIES AND THERAPEUTICS
• 影响因子: 3.1
• 作者: Abkallo, Hussein Molu;Kawano, Hiroaki;Kobayashi, Nobuyuki
• 通讯作者: Kobayashi, Nobuyuki