Inhibitor development and the functional elucidation of the drug efflux transporter which participates in multidrug resistance and pathogenic revelation

目前临床领域出现了一些耐药菌株，由这些菌株引起的传染病已成为一个主要问题。基因组分析已经鉴定出许多被认为是药物外排泵的基因，细菌基因组序列使我们能够鉴定细菌的耐药基因文库。根据沙门氏菌的基因组信息，我们已经鉴定出许多多药转运基因。此外，我们还发现了这些药物外排转运体在细菌毒力中的作用。在这项研究中，我们成功地发现了多药外排转运体调节细菌耐药性和毒力表型的机制。由于药物外排转运体在细菌多药耐药和毒力中发挥作用，我们认为药物外排转运体具有比通常认为的更大的临床相关性。

A number of drug-resistant bacterial strains are now appearing in the clinical field, and infectious diseases originating from these strains have become a major problem. Genomic analysis has resulted in the identification of many genes which have been proposed as drug efflux pumps, and bacterial genome sequences have allowed us to identify the drug-resistant gene libraries of bacteria. We have identified a number of multidrug transporter genes in Salmonella based on its genomic information. Moreover, we found the roles of these drug efflux transporters in bacterial virulence. In this study, we have succeeded in discovering the mechanism which regulate both bacterial drug resistance and virulence phenotypes modulated by the multidrug efflux transporters. Because drug efflux transporters have roles in bacterial multidrug resistance and virulence, we propose that drug efflux transporters have greater clinical relevance than is usually attributed to them.