Regulation of intra- or extracellular glucocorticoid concentration by Lipocalin2 as a candidate marker for steroid dosage.

本研究的目的是检验脂质运载蛋白2与糖皮质激素结合，并调节细胞内或细胞外游离糖皮质激素浓度的假设。为了验证这一假设，我们建立了一种利用表面等离子体共振系统检测脂质运载蛋白-2与糖皮质激素相互作用的方法。我们的表面等离子体共振分析表明，脂质运载蛋白-2与地塞米松的相互作用呈剂量依赖性，而脂质运载蛋白-2与泼尼松龙的相互作用没有检测到。这些结果表明，脂质运载蛋白-2有可能与特异性糖皮质激素结合。我们将研究脂质运载蛋白-2的生理作用。

The aim of this study is to examine the hypothesis that lipocalin-2 binds to glucocorticoids, and regulates the concentration of intra- or extracellular free glucocorticoids. To examine the hypothesis, we established an assay for interaction between lipocalin -2 and glucocorticoids using surface plasmon resonance system. Our surface plasmon resonance analysis showed that lipocalin-2 interacts with dexamethasone dose-dependently , while the interaction of lipocalin-2 with prednisolone was not detected.. These results suggested that lipocalin-2 has a possibility of binding to specific glucocorticoids. We will examine the physiological role of lipocalin-2.