Assessment of utility of saliva for lifestyle related disease screening

唾液在生活方式相关疾病筛查中的效用评估

• 批准号: 23790204
• 负责人: IWASAKI Yusuke
• 金额: $ 1万
• 依托单位: Hoshi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790204/
• 关键词: 酸化ストレス; 糖尿病; 唾液

An automated online solid-phase extraction method for the determination of the reduced and oxidized forms of thiols in mouse serum was developed and validated. Analysis was performed with column-switching hydrophilic interaction chromatography coupled with mass spectrometry (CS-HILIC-MS). The proposed CS-HILIC-MS method enabled the simultaneous determination of reduced and oxidized thiols in mouse serum samples. In addition, interference from endogenous compounds was removed by means of the column-switching technique. We also compared the effects of derivatization before and after preparing serum from blood samples and found that it was necessary to perform the derivatization immediately before preparing serum from blood samples. Serum reduced and oxidaized glutathione levels were significantly increased in diabetes mice. Our method is expected to be useful for the assessment of the roles of reduced and oxidized glutathione in the oxidative state.

建立了一种自动化在线固相萃取法测定小鼠血清中巯基的还原和氧化形式。采用柱切换亲水相互作用色谱-质谱联用（CS-HILIC-MS）进行分析。所提出的CS-HILIC-MS方法能够同时测定小鼠血清样品中的还原和氧化硫醇。此外，内源性化合物的干扰通过柱切换技术去除。比较了制备血清前后衍生化的效果，发现制备血清前立即进行衍生化是必要的。糖尿病小鼠血清还原型和氧化型谷胱甘肽水平显著升高。我们的方法预计将是有用的还原型和氧化型谷胱甘肽在氧化状态的作用的评估。

项目成果

Quantification of reduced and oxidized thiols in mouse serum by column-switching hydrophilic interaction chromatography coupled with mass spectrometry.

通过柱切换亲水相互作用色谱法与质谱联用对小鼠血清中还原和氧化硫醇进行定量。

• 发表时间: 2011
• 期刊: J Pharm Biomed Anal.
• 作者: Iwasaki Y;Nakano Y;Mochizuki K;Ogawa T;Oda M;Ito R;Saito K;Nakazawa H.
• 通讯作者: Nakazawa H.

酸化および窒素化ストレスマーカー分析のための試料前処理方法の検討

氧化和氮应激标记物分析的样品预处理方法检查

• 发表时间: 2012
• 作者: K. Kawano;Y. Hattori;H. Iwakura;T. Akamizu and Y. Maitani;Yohei Numasawa;岩崎雄介
• 通讯作者: 岩崎雄介