Identification and functional analysis of novel interacting proteins for regulation of mitochondrial c-Src activity
调控线粒体 c-Src 活性的新型相互作用蛋白的鉴定和功能分析
基本信息
- 批准号:23790341
- 负责人:
- 金额:$ 2.75万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Young Scientists (B)
- 财政年份:2011
- 资助国家:日本
- 起止时间:2011 至 2012
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Non-receptor-type tyrosine kinase c-Src is implicated in fundamental cellular functions including proliferation, differentiation and survival. Recent studies have suggested that c-Src is also present in mitochondria and involved in the regulatory mechanisms of mitochondrial energy production. Here we have identified novel interaction proteins with c-Src in mitochondria, and investigated their function in the regulation of c-Src kinase activity. Serum starvation exhibits significant reductions of respiration and mitochondrial c-Src activity, and an enhancement of reactive oxygen species production in human T98G cells. Proteome analysis reveals mitochondrial c-Srcinteraction proteins including ATP5A1 of respiratory complex V. Overexpression of ATP5A1 in cells exhibits a significant increase in c-Src kinase activity and respiration in mitochondria. These results suggest that ATP5A1 regulates c-Src kinase activity by its interaction with c-Src complex in mitochondria, and that their interaction is essential for cellular respiration.
非受体型酪氨酸激酶c-Src与增殖、分化和生存等基本细胞功能有关。最近的研究表明,c-Src也存在于线粒体中,并参与线粒体能量产生的调节机制。在这里,我们已经确定了新的相互作用蛋白质与c-Src在线粒体中,并研究其功能的c-Src激酶活性的调节。血清饥饿会显着降低人T98 G细胞的呼吸和线粒体c-Src活性,并增强活性氧的产生。蛋白质组分析显示线粒体c-Src相互作用蛋白包括呼吸复合体V的ATP 5A 1。ATP 5A 1在细胞中的过表达表现出c-Src激酶活性和线粒体呼吸的显着增加。这些结果表明,ATP 5A 1通过与线粒体中的c-Src复合物相互作用来调节c-Src激酶活性,并且它们的相互作用对于细胞呼吸是必需的。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Phosphorylation of respiratory complex I and II by c-Src is required for neuronal viability
c-Src 磷酸化呼吸复合物 I 和 II 是神经元活力所必需的
- DOI:
- 发表时间:2013
- 期刊:
- 影响因子:0
- 作者:Ogura M;Yamaki J;Homma MK;Kato S,Kobayashi K;Kobayashi K;Homma Y
- 通讯作者:Homma Y
Mitochondrial c-Src regulates cell survival through phosphorylation of respiratory chain components.
- DOI:10.1042/bj20120509
- 发表时间:2012-10-15
- 期刊:
- 影响因子:0
- 作者:Ogura M;Yamaki J;Homma MK;Homma Y
- 通讯作者:Homma Y
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