Characterization of malignant lymphoma based on factors that define cancer initiating cells

为了鉴定恶性淋巴瘤中的癌症起始细胞（cancer initiating cells，CIC），检测了霍奇金淋巴瘤细胞系中的醛脱氢酶（aldehyde dehydrogenase，ALDH）活性和活性氧（reactive oxygen species，ROS）的降解能力，所述活性氧已被报道在多种癌症中含有许多CIC。ROS-低和ALDH-高单核小尺寸细胞分别比ROS-高和ALDH-低细胞更有效地形成集落和肿瘤。FoxO 3a是一种调节ROS降解酶的转录因子，其表达在ROS低和ALDH高群体中均高于ROS高和ALDH低群体。这些结果表明，这些细胞可能在霍奇金淋巴瘤的肿瘤发生中起重要作用。

To identify cancer initiating cells (CICs) in malignant lymphoma, aldehyde dehydrogenase (ALDH) activities and degradation capability of reactive oxygen species (ROS), which have been reported to contain many CICs in several cancers, was examined in Hodgkin lymphoma cell lines. ROS-low and ALDH-high single-nucleated small sized cells formed colonies and tumors more efficiently than ROS-high and ALDH-low cells, respectively. The expression of FoxO3a, a transcription factor regulating ROS-degrading enzymes, was higher in both ROS-low and ALDH-high populations than in ROS-high and ALDH-low populations. These findings suggest that such cells might play an important role in tumorigenesis of Hodgkin lymphoma.