Comprehensive analysis of the changesin role of cardiac progenitor cells during aging

衰老过程中心脏祖细胞作用变化的综合分析

• 批准号: 23790855
• 负责人: YOSHIDA Masashi
• 金额: $ 2.83万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790855/
• 关键词: 心臓内幹細胞; 老化; DNA マイクロアレイ; 分子生物学; DNAマイクロアレイ

Aims of this study are to clarify the changes in role of cardiac progenitor cells (CPCs) during aging and to improve cardiogenic potential of those CPCs. We found that CPCs from human infant heart already became senescent when compared to those from neonates. This fact indicates CPCs possess potential of progenitor cells, not stem cells. Then we also found that CPCs from neonates had higher cardiogenic activity and CPCs from infants had higher angiogenic activity. DNA microarray analysis revealed that down regulation of insulin-like growth factor-1 receptor lead CPCs to have cardiogenic to angiogenic potential. Our results suggest a functional significance of IGF1R-mediated physiological alteration of human CPC differentiation potential.

本研究旨在阐明心脏祖细胞（cardiac progenitor cells, CPCs）在衰老过程中作用的变化，并提高其成心潜能。我们发现，与新生儿相比，来自人类婴儿心脏的心肌细胞已经衰老。这一事实表明，cpc具有祖细胞的潜能，而不是干细胞。然后我们还发现新生儿的心肌细胞具有较高的心源活性，而婴儿的心肌细胞具有较高的血管生成活性。DNA微阵列分析显示，胰岛素样生长因子-1受体的下调导致CPCs具有心源性到血管生成的潜能。我们的结果提示igf1r介导的人CPC分化电位生理改变的功能意义。

项目成果

Both insulin-like growth factor-1 and -2 receptor control human cardiac progenitor proliferation and differentiation in children with congenital heart malformation

胰岛素样生长因子-1和-2受体均控制先天性心脏畸形儿童心脏祖细胞的增殖和分化

• 发表时间: 2011
• 作者: Kashima Y;Takahashi M;Kimata K;Itano N;Ikeda U;吉田賢司
• 通讯作者: 吉田賢司

Hedgehog interacting proteicontrols age-dependent angiogenic activity in human cardiac progenitor cells through insulin-like growth factor-1 receptor signaling

Hedgehog 相互作用蛋白通过胰岛素样生长因子 1 受体信号传导控制人心脏祖细胞中年龄依赖性血管生成活性

• 发表时间: 2012
• 作者: 柴田玲 大内乗有;室原豊明;吉田賢司
• 通讯作者: 吉田賢司

Increased Akt-mTOR Signaling in Lung Epithelium Is Associated with Respiratory Distress Syndrome in Mice

• DOI: 10.1128/mcb.00732-10
• 发表时间: 2011-03-01
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Ikeda, Hiroyuki;Shiojima, Ichiro;Komuro, Issei
• 通讯作者: Komuro, Issei

Hedgehog interacting protein controls age-dependent angiogenic activity in human cardiac progenitor cells through insulin-like growth factor-1 receptor signaling

Hedgehog 相互作用蛋白通过胰岛素样生长因子 1 受体信号传导控制人心脏祖细胞中年龄依赖性血管生成活性

• 作者: Kashima Y;Takahashi M;Itano N;Taniguchi S;Kimata K;Ikeda U;Masashi Yoshida
• 通讯作者: Masashi Yoshida

Transition in cardiogenic to angiogenic potential of human cardiac progenitor cells occurs with age

人类心脏祖细胞的心源性向血管生成潜力的转变随着年龄的增长而发生

• 发表时间: 2012
• 作者: Kashima Y;Takahashi M;Shiba Y;Itano N;Izawa A;Koyama J;Nakayama J;Taniguchi S;Kimata K;Ikeda U.;吉田賢司
• 通讯作者: 吉田賢司