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Effects of ionizing radiation on the pattern recognition receptors.

电离辐射对模式识别受体的影响。

基本信息

批准号：
23791383
负责人：
YOSHINO Hironori
金额：
$ 2.58万
依托单位：
Hirosaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2011
资助国家：
日本
起止时间：
2011 至 2012
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791383/
关键词：
放射線自然免疫樹状細胞パターン認識受容体レドックス制御

项目摘要

Dendritic cells (DCs) play an essential role in the immune system. This study investigated whether DCs from monocytes exposed to ionizing radiation can adequately respond to pathogen-derived components and proinflammatory cytokines. Human monocytes separated from buffy coats were exposed to x-rays and were then differentiated into DCs. DCs were stimulated by lipopolysaccharide (LPS) or proinflammatory cytokine mixture (MIX). The DCs from nonirradiated and X-irradiated monocytes showed maturation after LPS and MIX stimulation as confirmed by findings of surface antigen expression. Upon LPS stimulation, however, the expression levels of CD80 and CD83 on the DCs from the X-irradiated monocytes were lower than those of the DCs from the nonirradiated monocytes. Such reductions were not observed upon MIX stimulation. Similarly, an impairment of matrix metalloproteinase-9 and cytokine production was observed in the LPS-stimulated DCs from the X-irradiated monocytes, whereas these impairments were not observed upon MIX stimulation. The ability of DCs to stimulate T cells was lower in the irradiated group compared with the nonirradiated group despite the type of maturation stimulus. Thus, the present results suggest that the influence of X-irradiation on the maturation of DCs depends on the type of maturation stimulus used and that X-irradiation especially impairs the response of DCs to LPS.

树突状细胞（dc）在免疫系统中起着至关重要的作用。这项研究调查了暴露于电离辐射的单核细胞的dc是否能充分响应病原体来源的成分和促炎细胞因子。从褐皮中分离的人单核细胞暴露在x射线下，然后分化成dc。脂多糖（LPS）或促炎细胞因子混合物（MIX）刺激树突状细胞。未照射和x照射单核细胞的树突状细胞在LPS和MIX刺激后表现成熟，表面抗原表达的结果证实了这一点。然而，在LPS刺激下，x照射单核细胞的dc上CD80和CD83的表达水平低于未照射单核细胞的dc。在MIX增产试验中没有观察到这种降低。同样，在x射线照射的单核细胞中，在lps刺激下的dc中观察到基质金属蛋白酶-9和细胞因子产生的损伤，而在MIX刺激下没有观察到这些损伤。尽管成熟刺激的类型不同，与未照射组相比，照射组的dc刺激T细胞的能力较低。因此，目前的研究结果表明，x照射对树突状细胞成熟的影响取决于所使用的成熟刺激类型，x照射尤其会损害树突状细胞对LPS的反应。