Creation of a high efficient luciferase

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The emission reaction is inhibited by the emission reaction by the product inhibition caused by the production of oxyluciferin in firefly luciferase. In order to understand the mechanism of the product inhibition, I determine the crystal structure of H247A mutant which decreases the degree of the product inhibition. The crystal structure shows that the interaction of between N- and C- terminal domain is weaken by the structural change. Because the active site might open easily , it is difficult to occur the product inhibition.