Protein interaction analysis of circadian clock protein

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BMAL1 and CLOCK are the transcription factors of the circadian clock in mammals. The human BMAL1:CLOCK bHLH domain forms hetero or homo dimer and binds to E-box DNA. The hetero dimer of bHLH domain was more preferable to recognize the E-BOX DNA compared to the homo dimer based on the ITC analysis. Next we investigated the region related to the formation of the bHLH PAS-A domain of the human BMAL1:CLOCK hetero dimer. It is suggested that the bHLH domain is more important for the hetero dimer formation than the PAS-A domain.