The arterial and venous differentiation of endothelial cells in the forming of the cranial vasculatures of zebrafish

斑马鱼颅血管形成中内皮细胞的动静脉分化

• 批准号: 23659100
• 负责人: HITOMI Jiro
• 金额: $ 2.33万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659100/
• 关键词: 発生・分化; 脳; 脈管形成; 分子認識; イメージング; 発現制御; 脳血管; 細胞応答; 血管新生

The cranial vascular system of zebrafish was composed of three clusters of the fli1 positive angioblasts, of which the first cluster recognized as lateral dorsal artery or primitive internal carotid artery, and the second cluster assembling at the rostral-internal region to the optic capsule, and the third cluster arising among the lateral-ventral wall of hindbrain. The rostral and caudal clusters of arterial origins express the arterial maker hey 2, while the clusters arising among the lateral-ventral wall of hindbrain express the venous maker flt4. Shh was expressed in the adjacent region of arterial angioblast-clusters, which are suggested to determine arterial endothelial fate. Time-lapse movies imaging the arterial-venous differentiation also revealed that the branches of intercarotid artery are originated by arterial angioblast clusters at the rostral, and the branches of vertebral-basilar artery are derived from the venous angioblast clusters arising among the hindbrain.

斑马鱼脑血管系统由3簇fil 1阳性的成血管细胞组成，第1簇为外侧背动脉或原始颈内动脉，第2簇聚集在头内侧区至视神经囊，第3簇出现在后脑外侧腹壁。动脉起源的吻侧和尾侧簇表达动脉标记hey 2，而在后脑侧腹壁之间出现的簇表达静脉标记flt 4。Shh在动脉成血管细胞簇的邻近区域中表达，这被建议用于确定动脉内皮命运。动-静脉分化的延时摄影也显示颈动脉的分支起源于吻侧的动脉成血管细胞团，椎-基底动脉的分支起源于后脑中的静脉成血管细胞团。

项目成果

個体内における1細胞レベルでの遺伝子発現制御法の開発.

开发在单细胞水平上控制个体内基因表达的方法。

• 发表时间: 2014
• 作者: 木村英二;人見次郎
• 通讯作者: 人見次郎

マウス骨髄における成熟巨核球の血小板産生過程の解析

小鼠骨髓成熟巨核细胞血小板生成过程分析

• 发表时间: 2013
• 作者: 古和田周吾;磯貝純夫;村井一範;伊藤薫樹;遠山稿二郎;人見次郎;石田陽治
• 通讯作者: 石田陽治

Origin of the endothelial cells and mechanism generate the anatomical architecture of the functional vascular system for the brain and spinal cord

内皮细胞的起源和机制产生大脑和脊髓功能性血管系统的解剖结构

• 发表时间: 2012
• 作者: Isogai S;Saito E;Kimura E;Hitomi J
• 通讯作者: Hitomi J

固体内における１細胞レベルでの遺伝子発現制御法の開発

开发在个体的单细胞水平上控制基因表达的方法

• 发表时间: 2014
• 作者: 木村英二;人見次郎
• 通讯作者: 人見次郎

Chemokine signaling directs trunk lymphatic network formation along the preexisting blood vasculature.

• DOI: 10.1016/j.devcel.2012.01.011
• 发表时间: 2012-04-17
• 期刊: DEVELOPMENTAL CELL
• 影响因子: 11.8
• 作者: Cha, Young Ryun;Fujita, Misato;Butler, Matthew;Isogai, Sumio;Kochhan, Eva;Siekmann, Arndt F.;Weinstein, Brant M.
• 通讯作者: Weinstein, Brant M.