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Morphological and molecular biological analyses for the mechanism of vascular development in the embryogenesis

胚胎发生中血管发育机制的形态学和分子生物学分析

基本信息

批准号：
16390049
负责人：
HITOMI Jiro
金额：
$ 9.6万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

The morphological and molecular biological analyses for the vascular development showed us the following novel phenomena precisely.Renal and the related arteries vary in their level of origin and in their calibre, number and precise anatomical relationship to other structures. Studies of the origin and early development of these arteries have evoked sharp disputes. The ladder theory of Felix, which states that 'All the mesonephric arteries may persist ; from them are formed the phrenic, suprarenal, renal and internal spermatic arteries' has been generally quoted in the anatomical textbooks without rigorous verification for 100 years. In this study, we re-examined this theory by performing micro-injection of dye and resin into rat embryos. Our results revealed that most of the mesonephric arteries had degenerated before the metanephros started its ascent The definitive renal and the related arteries appeared as new branches from the gonadal artery and/or directly from the abdominal aort … More a to the para-aortic ridge. We also demonstrated that the route of venous drainage changed from the posterior cardinal vein to the inferior (caudal) vena cava.The lymphatic system has become the subject of great interest in recent years because of its important role in normal and pathological processes. Progress in understanding the origins and early development of this system, however has been hampered by difficulties in observing lymphatic cells in vivo and in performing defined genetic and experimental manipulation of the lymphatic system in currently available model organisms. However we could show that the optically clear developing zebrafish provides a useful model for imaging and studying lymphatic development, with a lymphatic system that shares many of the morphological, molecular and functional characteristics of the lymphatic vessels found in other vertebrates. Our results showed lymphatic endothelial cells of the thoracic duct arise from primitive veins through a novel and unexpected pathway. Less

血管发育的形态和分子生物学分析准确地显示了以下新现象：肾动脉和相关动脉的起源水平不同，其管径、数目和与其他结构的精确解剖关系也不同。对这些动脉的起源和早期发育的研究引发了尖锐的争议。Felix的阶梯理论认为，所有的中肾动脉都可以存在，从中形成了膈动脉、肾上动脉、肾动脉和精索内动脉。100年来，解剖学教科书中普遍引用了这一理论，但没有经过严格的验证。在这项研究中，我们通过对大鼠胚胎进行微量注射染料和树脂来重新检验这一理论。我们的结果表明，大多数中肾动脉在后肾开始上升之前就已经退化，最后的肾动脉和相关动脉作为新的分支出现在性腺动脉和/或直接来自腹主动脉的…。更多的是到腹主动脉旁的脊线。我们还证实了静脉引流的途径从主后静脉到下腔静脉(尾腔静脉)。近年来，淋巴系统因其在正常和病理过程中的重要作用而成为人们极感兴趣的主题。然而，在了解这一系统的起源和早期发展方面的进展一直受到在体内观察淋巴细胞以及在现有模式生物中对淋巴系统进行明确的遗传和实验操作的困难的阻碍。然而，我们可以证明，光学透明的发育斑马鱼为成像和研究淋巴发育提供了一个有用的模型，其淋巴系统与其他脊椎动物中发现的淋巴管的许多形态、分子和功能特征相似。我们的结果表明，胸腔导管的淋巴管内皮细胞通过一种新的和意想不到的途径来自原始静脉。较少