Long-term gene expression in primary cells by transposon and its application for cell therapy

表达治疗性蛋白的细胞移植是一种长期供应治疗性蛋白的有希望的方法。这种基因细胞疗法将是一种治疗慢性或先天性疾病的新方法。在本研究中，我们利用转座子转化，建立了能长期表达目标蛋白的原代间充质干细胞。这些细胞在分化后继续表达目标蛋白。此外，我们还可以展示利用dna结合蛋白控制转化中插入位点的可能方法。

Transplantation of cells expressing therapeutic protein is a promising way to supply such proteins for long time. Such gene-cell therapy would be a novel treatment for chronic or congenital disease. In this study, we could established primary mesenchymal stem cells with long-term expression of target protein by transformation using transposon. These cells kept expressing the target protein after differentiation. Moreover, we could show the possible method for controlling insertion site in transformation using DNA-binding protein.