Long-term gene expression in primary cells by transposon and its application for cell therapy

移植表达治疗性蛋白质的细胞是长期供应此类蛋白质的有前景的方法。这种基因-细胞疗法将成为治疗慢性或先天性疾病的一种新方法。本研究通过转座子转化的方法，建立了能够长期表达目的蛋白的原代间充质干细胞。这些细胞在分化后继续表达目的蛋白。此外，我们可以显示可能的方法来控制插入位点的转化中使用的DNA结合蛋白。

Transplantation of cells expressing therapeutic protein is a promising way to supply such proteins for long time. Such gene-cell therapy would be a novel treatment for chronic or congenital disease. In this study, we could established primary mesenchymal stem cells with long-term expression of target protein by transformation using transposon. These cells kept expressing the target protein after differentiation. Moreover, we could show the possible method for controlling insertion site in transformation using DNA-binding protein.