Establishment of non-invasive diagnostic method for CKD by using urinary exosomes.

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We isolated exosomes from diabetic nephropathy patients and examined the expression of podocyte-derived exosomal transcriptional factors (PDETFs). To determine the severity of kidney injuries, albuminuria and PDETFs were measured and analyses. While extent of albuminuria was not different among all nephrotic syndrome patients, progressing renal diseases such as diabetic nephropathy exhibited high levels of urinary PDETFs. Glomerular expression of PDETFs was decreased in accordance with the amount of PDETFs in urine in each diabetic nephropathy patients. Evaluation and analysis of PDETFs would provide some clues to understanding the molecular linkage between CKD and CVD.