Investigation for progressive factors of glomerulosclerosis.

肾小球硬化是导致终末期肾病的最重要因素。导致系膜细胞肥大和表型改变的机制是随后发生肾小球硬化的原因。然而，肾小球损伤的确切机制尚不完全清楚。一种新型的IV型胶原基因DNA结合蛋白(MSW)具有多种功能，包括大亚基DNA复制因子C和DNA结合血管紧张素原和促肾上腺皮质激素释放因子。我们发现MSW是一个转录因子，调节IV型胶原基因，并且MSW是系膜细胞平滑肌表型改变的重要调节器。基因表达的调控与MSW从细胞核到细胞质的转运有关。我们发现，转化生长因子-β是生活垃圾转运的重要调节器，最终调控基因表达。这些结果表明，MSW蛋白在血管平滑肌表型调控中起重要作用，并可能参与肾小球细胞增殖和肾小球硬化的发生发展。

Glomerulosclerosis is the most important leading to end-stage kidney disease. The mechanism leading to mesangial cell proleferation and phenotypic change is responsible for the subsequent development of glomeruloscierosis. However, the precise mechanism for glomerular injuries is not completely understood. A novel DNA binding protein (MSW) for type IV collagen gene has a multiple functions including large subunit of DNA replication factor C and DNA binding for angiotensinogen and pro-piomelanocortin corticotropin releasing factor. We found that MSW is a transcription factor, which regulates type IV collagen gene and that MSW is a important modulator in a smooth muscle phenotypic change of mesangial cells. The regulation of the gene expression is associated with translocation of MSW from nucleus to cytoplasm. We found that transforming growth factor-β is an important modulator for the translocation of MSW, which eventually regulates the gene expression. These results indicate that MSW protein plays an important role in regulation of smooth muscle phenotype and may contribute to glomerular cell proliferation and the development of glomerulosclerosis.