Analysis of gene expression profiles in inflammatory skin disorders

为了将激光显微解剖的分析应用于皮肤病，我们首先比较了下表皮和上表皮中的mRNA表达。当我们检测只在颗粒层中表达的代表性基因--微丝蛋白mRNA的表达时，激光显微切割分析清楚地表明，微丝蛋白在上表皮中的表达比在下表皮中更丰富。接下来，我们展示了角化过程中与铁排泄相关的mRNA从细胞内到细胞外的增强表达，这与铁在表皮中的分布相一致。最后，我们检测了免疫相关基因RANKL在乳房外Paget病中的表达。在含有Paget细胞的表皮中，RANKL mRNA的表达高于正常表皮

To apply the analysis using laser microdissection to skin diseases, we first compared mRNA expression between the lower and upper epidermis. When we examined filaggrin mRNA expression as a representative gene expressed only in the granular layer, the analysis using laser microdissection clearly demonstrated more abundant expression of filaggrinmRNA in the upper epidermis than in the lower epidermis. Next, we demonstrated augmented expression of mRNA related with the excretion of iron from inside to outside of the cell in the course of keratinization, which corresponds with the iron distribution in the epidermis. Finally, we examined immune-related gene, RANKL mRNA expression in extramammary Paget's disease. We could demonstrated more RANKL mRNA in the epidermis containing Paget's cells than the normal epidermis