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Analysis of biological response of epidemal components to simple chemicals

表皮成分对简单化学品的生物反应分析

基本信息

批准号：
10670776
负责人：
AIBA Setsuya
金额：
$ 2.37万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670776/
关键词：
Dendritic cells Contact dermatitis Hapter Cytokine 抗原提示アポトーシス

项目摘要

As is well known in the case of Langerhans cells, dendritic cells (DCs) play a crucial role in the initiation of immunity to simple chemicals such as noted in the contact hypersensitivity. Since DCs are scattered in nonlymphoid organs as immature cells. They must be activated to initiate primary antigen specific immune reactions. Therefore, we hypothesized that some simple chemicals must affect the function of DCs. In this paper, we first demonstrated that human monocyte-derived DCs responded to such simple chemicals as DNCB, TNCB, DNFB, NiClィイD22ィエD2, MnClィイD22ィエD2, CoClィイD22ィエD2, SnClィイD22ィエD2, and CdSOィイD24ィエD2 by augmenting their expression of CD86 or HLA-DR antigen, down-regulating c-Fms expression or increasing their production of TNFα. In addition, the DCs stimulated with the chemicals demonstrated increased allogeneic T cell stimulatory function. Next, we found that, among these chemicals, only NiClィイD22ィエD2 and CoClィイD22ィエD2 induced apoptosis in them. Finally, we examined the effects of these chemicals on CD86 expression by 3 different macrophage subsets and DCs induced from the cultures of human peripheral blood monocytes in the presence of M-CSF, M-CSF + IL-4, GM-CSF, and GM-CSF + IL-4, respectively. Among them, only DCs dramatically augmented their expression of CD86. These observations have revealed unique characteristics of DCs which convert chemical stimuli to augmentation of their antigen presenting function, although their responses to different chemicals were not necessarily uniform in the phenotypic changes cytokine production or in the induction of apoptosis.

众所周知，在朗格汉斯细胞的情况下，树突状细胞（dc）在启动对简单化学物质的免疫方面起着至关重要的作用，例如接触性超敏反应。因为树突状细胞作为未成熟细胞分散在非淋巴器官中。它们必须被激活以启动初级抗原特异性免疫反应。因此，我们假设一些简单的化学物质一定会影响dc的功能。在本文中,我们首先证明了人类monocyte-derived DCs对DNCB等简单的化学物质,TNCB, DNFB, NiClィイD22摊位ィエD2, MnClィイD22摊位ィエD2, CoClィイD22摊位ィエD2, SnClィイD22摊位ィエD2,和CdSOィイD24ィエD2通过增加CD86的表达或HLA-DR抗原,显示c-Fms表达式或增加他们的肿瘤坏死因子α的生产。此外，化学物质刺激的dc表现出增强的同种异体T细胞刺激功能。接下来，我们发现在这些化学物质中，只有NiCl D22 D2和CoCl D22 D2诱导细胞凋亡。最后，我们分别在M-CSF、M-CSF + IL-4、GM-CSF和GM-CSF + IL-4的存在下，检测了这些化学物质对人外周血单核细胞培养的3种不同巨噬细胞亚群和DCs中CD86表达的影响。其中，只有dc显著增强了CD86的表达。这些观察结果揭示了树突状细胞的独特特征，它们将化学刺激转化为增强其抗原呈递功能，尽管它们对不同化学物质的反应在表型变化、细胞因子产生或诱导凋亡方面不一定是一致的。