Analysis of biological response of epidemal components to simple chemicals

在Langerhans细胞的情况下众所周知， Dendritic细胞（DC）在免疫性的启动中扮演一个简单化学品的规则角色，如在接触超敏感性中注意到的那样。自从DC被分散在非淋巴瘤器官中，就像不成熟的细胞一样。它们必须被激活以启动初级抗原特定免疫反应。因此，我们假设一些简单的化学品必须影响直流电的功能。在本文中，我们第一次演示了人类单细胞衍生的DCs响应于这样的简单化学品为DNCB、TNCB、DNFB、NiClIIID 22 ID 2、MnClID 22 ID 2、CoClID 22 ID 2、SnClID 22 ID 2和CdSO ID 24 ID 2由增强其表达的CD 86或HLA-DR antigen、向下调节c-FMS表达或增加TNFα的产物。在添加中，DCs被化学品演示增强了对称T细胞刺激功能。下一步，我们找到了，在这些化学品中，只有NiCl D22和CoCl D22诱导的凋亡。最后，我们研究了CD 86表达的3种不同宏观成分和直流化合物对CD 86化学品的影响，这些化学品从人类周边血液单体的文化中吸取了M-CSF、M-CSF + IL-4、GM-CSF + IL-4和GM-CSF + IL-4的存在中，对此表示赞赏。在他们中间，只有DC戏剧性地增强了CD 86的表达。这些观察揭示了DC的独特特征，其中转换化学刺激增强了其抗原表达功能的作用，但他们对不同化学品在表型变化细胞激素生产或凋亡的诱导中不必要统一的反应。

As is well known in the case of Langerhans cells, dendritic cells (DCs) play a crucial role in the initiation of immunity to simple chemicals such as noted in the contact hypersensitivity. Since DCs are scattered in nonlymphoid organs as immature cells. They must be activated to initiate primary antigen specific immune reactions. Therefore, we hypothesized that some simple chemicals must affect the function of DCs. In this paper, we first demonstrated that human monocyte-derived DCs responded to such simple chemicals as DNCB, TNCB, DNFB, NiClィイD22ィエD2, MnClィイD22ィエD2, CoClィイD22ィエD2, SnClィイD22ィエD2, and CdSOィイD24ィエD2 by augmenting their expression of CD86 or HLA-DR antigen, down-regulating c-Fms expression or increasing their production of TNFα. In addition, the DCs stimulated with the chemicals demonstrated increased allogeneic T cell stimulatory function. Next, we found that, among these chemicals, only NiClィイD22ィエD2 and CoClィイD22ィエD2 induced apoptosis in them. Finally, we examined the effects of these chemicals on CD86 expression by 3 different macrophage subsets and DCs induced from the cultures of human peripheral blood monocytes in the presence of M-CSF, M-CSF + IL-4, GM-CSF, and GM-CSF + IL-4, respectively. Among them, only DCs dramatically augmented their expression of CD86. These observations have revealed unique characteristics of DCs which convert chemical stimuli to augmentation of their antigen presenting function, although their responses to different chemicals were not necessarily uniform in the phenotypic changes cytokine production or in the induction of apoptosis.