High-throughput screening for peptides that inhibit the interaction or MDM4 with p53

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It is currently of great midical interest to inhibit specific protein-protein interactions between MDM4 and p53. Here, we developed a method using T7 phage display to screen for candidate inhibitors of MDM4-p53 interactions. In primary screening , we identified novel peptide motifs that inhibit the interaction of MDM4 with p53. Then, we designed a number of peptides that are predicted to have higher affinity to MDM4, and identified potential peptides that inhibit the interaction between MDM4 and p53 with higher affinity.