Engineering enzymes controlling plant polysaccharide properties

控制植物多糖特性的工程酶

• 批准号: DE150100837
• 负责人: Prof Caitlin Byrt
• 金额: $ 24.38万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-06-30 至 2018-06-30
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE150100837
• 关键词: Engineering; enzymes; controlling; plant; polysaccharide

This project will aim to use data to define how the synthesis and interconversion of nucleotide sugars is regulated and how this controls the properties of arabinoxylan in economically important plants. Dietary consumption of arabinoxylan reduces chronic diseases. Additionally, the attributes of arabinoxylan influence the cost of processing plant biomass. However, genetic control of the properties of the plant polysaccharide arabinoxylan is unresolved. A major control point in the partitioning of carbon from photosynthesis into arabinoxylan is the activity of sugar nucleotide interconverting enzymes. To characterise these enzymes, genomic, glycomic and enzyme kinetic data will be combined and the target enzymes will be modified in transgenic plants.

该项目旨在利用数据来确定核苷酸糖的合成和相互转化是如何被调节的，以及这是如何控制经济上重要植物中阿拉伯木聚糖的特性的。饮食中摄入阿拉伯木聚糖可减少慢性疾病。此外，阿拉伯木聚糖的属性影响加工植物生物量的成本。然而，植物多糖阿拉伯木聚糖特性的遗传控制尚未得到解决。碳从光合作用分配到阿拉伯木聚糖的一个主要控制点是糖核苷酸互转换酶的活性。为了表征这些酶，基因组学、糖糖学和酶动力学数据将结合起来，目标酶将在转基因植物中进行修饰。