mechanism of bile duct carcinogenesis using rat model of pancreaticobiliary maljunction

胰胆管异常时，胰液反流可引起许多疾病，包括炎症，并可能与癌变有关。然而，其机制尚不清楚。这次我们给DBTC torats，并制作胰胆管畸形模型。单剂量8mg/kg DBTC可引起胆道淤积和上皮乳头状生长，免疫组化证实Ki-67、COX2和HDAC高表达。然而，给药后4周乳头生长恢复。由于大鼠因频繁给药而死亡，且无法长时间观察，我们尝试采用手术技术制作小鼠胰胆异常模型。

In pancreaticobiliary maljunction, reflux of pancreatic juice causes many conditions including inflammation, and may be relate to carcinogenesis. However, mechanism is not well known. This time, we administered DBTC torats and made the model of pancreaticobiliary maljunction. Single dose of 8mg/kg DBTC caused biliary stasis and papillary growth of epithelium, and we confirmed high expression of Ki-67, COX2 and HDAC by immunohistochemistry. However, papillary growth was recovered 4 weeks after administration. As Rats died by frequent dose of DBTC and we could not observe in long time, we try to make mouse model of pancreaticobiliary maljunction by surgical technique.