mechanism of bile duct carcinogenesis using rat model of pancreaticobiliary maljunction

在胰胆管连接不良中，胰液回流可引起包括炎症在内的多种情况，并可能与肿瘤的发生有关。然而，其机制还不是很清楚。这一次，我们实施了DBTC扭伤，并制作了胰胆管连接不良模型。单次给药8 mg/kg可引起胆汁淤积和上皮乳头状生长，免疫组织化学证实Ki-67、COX2和HDAC高表达。给药4周后，乳头状肌生长恢复正常。由于DBTC频繁给药致大鼠死亡，且不能长期观察，我们尝试用外科技术制作小鼠胰胆管合流不良模型。

In pancreaticobiliary maljunction, reflux of pancreatic juice causes many conditions including inflammation, and may be relate to carcinogenesis. However, mechanism is not well known. This time, we administered DBTC torats and made the model of pancreaticobiliary maljunction. Single dose of 8mg/kg DBTC caused biliary stasis and papillary growth of epithelium, and we confirmed high expression of Ki-67, COX2 and HDAC by immunohistochemistry. However, papillary growth was recovered 4 weeks after administration. As Rats died by frequent dose of DBTC and we could not observe in long time, we try to make mouse model of pancreaticobiliary maljunction by surgical technique.