The role of the gene bozooka during asymmetric division of neural stem cells in Drosophila

bozooka基因在果蝇神经干细胞不对称分裂中的作用

• 批准号: 5415887
• 负责人: Professor Dr. Andreas Wodarz
• 金额: --
• 依托单位: Institut I für Anatomie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2003
• 资助国家: 德国
• 起止时间: 2002-12-31 至 2005-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5415887?language=en
• 关键词: role; gene; bozooka; during; asymmetric

Typical stem cell divisions are asymmetric and produce two daughter cells different developmental potential. In most cases, one daughter cell adopts a progenitor fate and will produce cells that undergo terminal differentiation, while the other daughter cell maintains the full potential of a stem cell. The stem cells of the Drosophila central nervous system, so called neuroblasts, divide asymmetrically and give rise to a ganglion mother cell (a neuronal progenitor cell) and another neuroblast in each division. We have identified two genes, bazooka and DaPKC, that are indispensable for asymmetric localization of cell fate determinants and for correct orientation of the mitotic spindle in neuroblasts. Both genes encode core components of the evolutionarily highly conserved PAR/aPKC complex. In the proposed project we investigate which regions of the Bazooka protein are functionally important for the control of asymmetric neuroblast division. To this aim, we assay the subcellular localization and functionality of several mutant versions of Bazooka generated by in vitro mutagenesis in vivo using transgenic flies. In addition, we use a dominant overexpression phenotype in the eye as a readout for a genetic screen to isolate point mutations in bazooka and to identify genes that functionally interact with bazooka.

典型的干细胞分裂是不对称的，并产生两个子细胞不同的发育潜力。在大多数情况下，一个子细胞采用祖细胞的命运，并将产生经历终末分化的细胞，而另一个子细胞保持干细胞的全部潜力。果蝇中枢神经系统的干细胞，即所谓的神经母细胞，不对称分裂，每次分裂产生一个神经节母细胞（神经元祖细胞）和另一个神经母细胞。我们已经确定了两个基因，bazooka和DaPKC，这是不可缺少的不对称定位的细胞命运的决定因素和正确的方向的有丝分裂纺锤体在成神经细胞。这两个基因编码进化上高度保守的PAR/aPKC复合物的核心成分。在拟议的项目中，我们调查的Bazooka蛋白的哪些区域是功能上重要的控制不对称的神经母细胞分裂。为了这个目的，我们分析了几个突变版本的巴祖卡在体内使用转基因苍蝇体外诱变产生的亚细胞定位和功能。此外，我们使用一个显性过表达表型的眼睛作为读出的基因筛选，以隔离点突变的火箭筒和识别基因的功能与火箭筒相互作用。