Dynamic Force Microscopy of small molecular assemblies.

操控单个分子的可能性一开始似乎是不现实的，事实上，5年前这还是纯粹的科幻小说。随着原子力显微镜(AFM)的普及，人们对分子世界的许多看法正在发生变化。现在，根据用该仪器进行的测量，讨论粘附性和润滑性等宏观效应。更新的工作包括观察单个蛋白质的展开实验。本申请中详细介绍的面向生物物理学的项目将把原子力显微镜扩展到通过自组装形成的多分子系统，如细胞膜；聚电解质-表面相互作用；最后，DNA测序。

The possibility of manipulating a single molecule seems at first unreal, indeed 5 years ago it was pure science fiction. Through the gaining popularity of the Atomic Force Microscope (AFM) many perspectives about the molecular world are changing. Macroscopic effects such as adhesion and lubrication are now discussed in light of measurements made with this instrument. Newer work includes the observation of single protein unfolding experiments. The biophysics oriented project detailed in this application will extend the AFM: into multi-molecular systems formed by self-assembly, such as cell membranes; into polyelectrolyte-surface interactions; and, finally into the sequencing of DNA.