Characterisation and evolution of chromosome complements of carnivorous Lentibulariaceae

肉食性扁豆科染色体互补体的特征和进化

• 批准号: 212721201
• 负责人: Professor Dr. Ingo Schubert
• 金额: --
• 依托单位: Abteilung Züchtungsforschung
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/212721201?language=en
• 关键词: Characterisation; evolution; chromosome; complements; carnivorous

The cosmopolitan Lentibulariaceae family is characterized by extreme plasticity of genome size, variable chromosome numbers, high base substitution rates and unique trapping mechanisms for carnivory. Genomes and chromosome complements of this interesting plant family are only scarcely studied. For Genlisea pygmaea. (76 Mbp; n=15) with one of the smallest plant genomes, for G. margaretae (175 Mbp; n=18 or 19) and for G. hispidula (1530 Mbp, n=17 or 18), the basic ploidy level (diploidy vs. polyploidy), the centromere structure (monocentric vs. polycentric), the chromosomal constitution regarding heterochromatin, telomeres, and rDNA as well as the subnuclear distribution of epigenetic markers will be determined. A BAC library will be established, ordered into a tiling path and used to elucidate karyotype evolution, in particular the reasons of chromosome number alteration, within the genus Genlisea and potentially other Lentibulariaceae species via comparative chromosome painting. It is aimed to find out, whether similar or alternative mechanisms shaped Lentibulariaceae karyotypes, compared to the situation described for other seed plant families. Reciprocal painting with mapped and sequenced BAC pools will reveal the degree of synteny to A. thaliana, a member of the Brassicaceae family.

广泛分布的香菇科植物具有基因组大小可塑性强、染色体数目可变、碱基替换率高和独特的捕集机制等特点。对这一有趣植物科的基因组和染色体补体的研究很少。对于Genlisea pygmaea。（76 Mbp, n=15），其中最小的植物基因组之一，对于G. margaretae （175 Mbp, n=18或19）和G. hispidula (1530 Mbp, n=17或18)，基本倍性水平（二倍体与多倍体），着丝粒结构（单中心与多中心），染色体结构有关异染色质，端粒和rDNA以及表观遗传标记的亚核分布将被确定。将建立一个BAC文库，按顺序排列，并通过比较染色体绘制来阐明Genlisea属和其他可能的Lentibulariaceae物种的核型进化，特别是染色体数目改变的原因。与其他种子植物科的情况相比，目的是找出是否类似或替代机制形成了扁桃科的核型。与已绘制和测序的BAC池的互反绘画将揭示与芸苔科成员a . thaliana的同源程度。

项目成果

Comparative Genome Analysis Reveals Divergent Genome Size Evolution in a Carnivorous Plant Genus

• DOI: 10.3835/plantgenome2015.04.0021
• 发表时间: 2015-11
• 期刊: The Plant Genome
• 作者: G. H. Vu;Thomas Schmutzer;F. Bull;H. X. Cao;J. Fuchs;Trung D. Tran;G. Jovtchev;K. Pistrick;N. Stein;A. Pečinka;P. Neumann;P. Novák;J. Macas;P. Dear;F. Blattner;U. Scholz;I. Schubert

Centromere and telomere sequence alterations reflect the rapid genome evolution within the carnivorous plant genus Genlisea.

着丝粒和端粒序列的改变反映了食虫植物属内基因组的快速进化

• DOI: 10.1111/tpj.13058
• 发表时间: 2015
• 期刊: The Plant journal : for cell and molecular biology
• 作者: Trung D. Tran;Hieu X. Cao;Gabriele Jovtchev;Pavel Neumann;Petr Novák;Miloslava Fojtová;Giang T.H. Vu;Jiří Macas;Jiří Fajkus;Ingo Schubert;Joerg Fuchs
• 通讯作者: Joerg Fuchs