Development of a rapid GC-MS analysis method for drugs in blood using solid-phase microextraction and high temperature vaporization.

利用固相微萃取和高温汽化开发血液中药物的快速 GC-MS 分析方法。

• 批准号: 24790647
• 负责人: WATERS Brian
• 金额: $ 2.66万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790647/
• 关键词: 乱用薬物; 覚せい剤; バルビツレート; ベンゾジアゼピン; ヘッドスペース; 固相マイクロ抽出; ガスクロマトグラフィー; Drugs of abuse; Amphetamines; Barbiturates; Benzodiazepines; Headspace; SPME; Fast GC-MS/MS; methamphetamine; amphetamine; headspace; fast GC-MS/MS

In this study, a rapid method for analyzing a number of drugs of abuse from whole blood was developed. The goal was to accomplish extraction of the compounds while avoiding harmful organic solvents and tedius solid-phase extraction media. Solid-phase microextraction (SPME) techniques were employed to accomplish this. Initially, the focus was on amphetamine and methamphetamine. Analysis was achieved with pre-treatment of 0.1 g of sample with 0.1 ml of a strong base (5N NaOH). It was also determined that heating the headspace to a temperature of 220C was ideal for adsorption to the SPME fiber. Finally, the exposure time of the fiber to the heated headspace was examined. It was determined that exposure of the SPME fiber to the headspace for 7 minutes was ideal. These conditions were then used to analyze barbiturates and benzodiazepines in whole blood, however, analysis by SPME was problematic. Further study of SPME analysis of these classes of drugs in whole blood is necessary.

在这项研究中，开发了一种快速分析全血中滥用药物的方法。目标是在避免有害有机溶剂和单调的固相萃取介质的同时完成化合物的萃取。固相微萃取（SPME）技术来实现这一点。最初，重点是安非他明和甲基安非他明。用0.1 ml强碱（5 N NaOH）预处理0.1 g样品进行分析。还确定将顶部空间加热至220 ℃的温度对于吸附至SPME纤维是理想的。最后，检查纤维暴露于加热的顶部空间的时间。确定SPME纤维暴露于顶部空间7分钟是理想的。然后使用这些条件分析全血中的巴比妥类药物和苯二氮卓类药物，然而，SPME分析存在问题。有必要进一步研究全血中这几类药物的SPME分析。

项目成果

Optimization of a Method for the Quantification of Underivatized Amphetamines in Whole Blood Using Headspace Solid-Phase Microextraction and Fast GC-MS/MS

使用顶空固相微萃取和快速 GC-MS/MS 定量全血中未衍生化安非他明的方法优化

• 发表时间: 2013
• 作者: Brian Waters;Kenji Hara;Masayuki Kashiwagi;Aya Matsusue;Mio Takayama;Hiroshi Fujii;Shin-ichi Kubo
• 通讯作者: Shin-ichi Kubo

Fujii Hiroshi, Kubo Shin-ichi, Optimization of a Method for the Quantification of Underivatized Amphetamines in Whole Blood Using Headspace Solid-Phase Microextraction and Fast GC-MS/MS

Fujii Hiroshi、Kubo Shin-ichi，利用顶空固相微萃取和快速 GC-MS/MS 优化全血中未衍生化安非他明的定量方法

• 发表时间: 2013
• 作者: WATERS Brian;Hara Kenji;Kashiwagi Masayuki;Matsusue Aya;Takayama Mio
• 通讯作者: Takayama Mio

A fast and simple method for analyzing drugs of abuse in whole blood using high temperature vaporization and solid-phase microextraction

利用高温汽化和固相微萃取分析全血中滥用药物的快速而简单的方法

• 发表时间: 2014
• 作者: WATERS Brian;Fujii Hiroshi;Hara Kenji;Matsusue Aya;Kashiwagi Masayuki;Kubo Shin-ichi
• 通讯作者: Kubo Shin-ichi

A fast and simple method for analyzing drugs of abuse in whole blood using high temperature vaporization and solid-phase microextraction.

一种利用高温汽化和固相微萃取分析全血中滥用药物的快速而简单的方法。

• 发表时间: 2014
• 作者: Brian Waters;Hiroshi Fujii;Kenji Hara;Aya Matsusue;Masayuki Kashiwagi;Shin-ichi Kubo
• 通讯作者: Shin-ichi Kubo