Development of a rapid GC-MS analysis method for drugs in blood using solid-phase microextraction and high temperature vaporization.

本研究建立了一种从全血中快速分析多种滥用药物的方法。其目的是在避免有害有机溶剂和繁琐的固相萃取介质的情况下完成化合物的提取。固相微萃取(SPME)技术被用来完成这一过程。最初，重点是苯丙胺和甲基苯丙胺。用0.1ml强碱(5N NaOH)对0.1g样品进行前处理即可完成分析。还确定了将顶空加热到220℃是吸附SPME纤维的理想条件。最后，对纤维在加热顶空中的暴露时间进行了测试。确定将SPME纤维暴露在顶腔7分钟是理想的。这些条件被用来分析全血中的巴比妥酸盐和苯二氮卓类药物，然而，SPME的分析是有问题的。进一步开展全血中这类药物的固相微萃取分析研究是十分必要的。

In this study, a rapid method for analyzing a number of drugs of abuse from whole blood was developed. The goal was to accomplish extraction of the compounds while avoiding harmful organic solvents and tedius solid-phase extraction media. Solid-phase microextraction (SPME) techniques were employed to accomplish this. Initially, the focus was on amphetamine and methamphetamine. Analysis was achieved with pre-treatment of 0.1 g of sample with 0.1 ml of a strong base (5N NaOH). It was also determined that heating the headspace to a temperature of 220C was ideal for adsorption to the SPME fiber. Finally, the exposure time of the fiber to the heated headspace was examined. It was determined that exposure of the SPME fiber to the headspace for 7 minutes was ideal. These conditions were then used to analyze barbiturates and benzodiazepines in whole blood, however, analysis by SPME was problematic. Further study of SPME analysis of these classes of drugs in whole blood is necessary.