Demelopment of a realtime PCR-based semen identification method using the semen-specific hypomethylated DNA region

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In this study, we analyzed aged stains of various body fluids by bisulfite sequence analysis for the methylation status in the previously reported semen-specific hypomethylated region, DACT1. The results indiate that the methylation status is stable in each old stain and that this can be a useful marker for forensic semen identification. We further developed a TaqMan realtime PCR detection method to make it easier to analyze the methylation status of DACT1 region. This method can be a useful supplementary tool for semen identification, especially from aged stains.