A study on the function of Sphingomyelin Synthases

鞘磷脂合成酶的功能研究

基本信息

  • 批准号:
    18570143
  • 负责人:
  • 金额:
    $ 2.46万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2006
  • 资助国家:
    日本
  • 起止时间:
    2006 至 2007
  • 项目状态:
    已结题

项目摘要

Osteocytes are thought to play crucial roles in bone metabolism, although limited information has been available on their specific products and physiological function. To isolate genes expressed in osteocytes, we employed subtractive suppression PCR method using RNA from osteocyte-enriched bone fraction. A genes isolated by the method encodes a novel enzyme involved in phospholipid metabolism, which has been found as a sphingomyelin synthase(SMS2 : Huitema, et. al.(2004) ; Yamaoka, et. al.(2004)). To determine the expression of Sms2 in vivo, LacZ gene was introduced into mouse Sms2 locus to monitor the expression of Sms2. At E13.5, the expression, as monitored by X-gal staining, was detected only in skeletal elements, especially in the areas enriched in osteoblasts. The expression was observed not only in osteoblasts but also in osteocytes of E16.5 embryo. Sms2 was expressed in some differentiated chondrocytes of long bones, but not detected in rib or articular cartilages which remain uncalcified. In adult, although the expression was detected in non-skeletal tissues, it is markedly expressed in osteocytes. It has been reported that sphingomyelinase activity was detected in matrix vesicles, which play a pivotal role in matrix mineralization, and that sphingomyelin was hydrolyzed upon mineralization. Furthermore, mice carrying mutations in SmpdS, a gene encoding neutral sphingomyelinase, exhibited skeletal malformation and delayed calcification. Indeed, it prompted us to hypothesize that sphingomyelin may have some inhibitory effects on mineralization. In fact, when Sms2 gene was overexpressed in osteoblasts, mineralization was significantly suppressed. Thus, Sms2 may play a role in terminal differentiation of osteoblast and/or matrix mineralization in bone.
骨细胞被认为在骨代谢中起着关键作用,尽管关于其特定产物和生理功能的信息有限。为了分离在骨细胞中表达的基因,我们使用了从富含骨细胞的骨组织中提取RNA的消减抑制聚合酶链式反应方法。用这种方法分离的A基因编码一种与磷脂代谢有关的新酶,它被发现是一种鞘磷脂合成酶(SMS2:Huitema,et.等(2004);Yamaoka等人。Al.(2004))。为了检测Sms2在体内的表达,将LacZ基因导入小鼠Sms2基因座,以监测Sms2的表达。在E13.5,X-Gal染色仅在骨骼元素中检测到该表达,尤其是在富含成骨细胞的区域。该基因不仅在成骨细胞中表达,而且在E16.5胚胎的骨细胞中也有表达。SMS2在长骨的部分分化软骨细胞中有表达,但在未钙化的肋骨或关节软骨中未见表达。在成人中,虽然在非骨骼组织中检测到该基因的表达,但在骨细胞中明显表达。有报道称,在基质矿化过程中起关键作用的基质小泡中检测到了鞘磷脂酶的活性,并且在基质矿化过程中,鞘磷脂发生了水解性反应。此外,携带SmpdS突变的小鼠表现出骨骼畸形和钙化延迟。SmpdS是一种编码中性鞘磷脂酶的基因。事实上,它促使我们假设神经鞘磷脂可能对矿化有一些抑制作用。事实上,当Sms2基因在成骨细胞中过表达时,矿化明显受到抑制。因此,Sms2可能在成骨细胞的终末分化和/或骨中基质的矿化中发挥作用。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Osteoporosis in Older Persons : Pathophysiology and Therapeutic Approach.
老年人骨质疏松症:病理生理学和治疗方法。
Sms2,a Sphingomyelin Synthase Gene Expressed in Calcifying Osteoblasts and Osteocytes.
Sms2,一种在钙化成骨细胞和骨细胞中表达的鞘磷脂合酶基因。
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ken Watanabe;et. al.
  • 通讯作者:
    et. al.
骨芽細胞系に発現するスフィンゴミエリン合成酵素Sms2
成骨细胞谱系中表达的鞘磷脂合酶 Sms2
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    渡辺 研;他
  • 通讯作者:
Sms2, a sphingomyelin synthase gene expressed in osteoblast lineage.
Sms2,一种在成骨细胞谱系中表达的鞘磷脂合酶基因。
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ishikura N;Shinotsuka C;Hishiya A;Ikeda K;Watanabe K
  • 通讯作者:
    Watanabe K
Sms2, a Sphingomyelin Synthase Gene Expressed in Calcifying Osteoblasts and Osteocytes.
Sms2,一种在钙化成骨细胞和骨细胞中表达的鞘磷脂合酶基因。
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ishikura N;Shinotsuka C;Hishiya A;Ikeda K;Watanabe K
  • 通讯作者:
    Watanabe K
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WATANABE Ken其他文献

みちのくの仏像と東北における仏像表現の受容
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  • 发表时间:
    2015
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    0
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    ABE Hisashi ;WATANABE Ken;ISHIKAWA Atsuko;NOSHIRO Shuichi;FUJII Tomoyuki;IWASA Mitsuharu;KANEKO Hiroyuki;WADA Hiroshi;山盛弥生;宮下 修一;村田雄二郎;Tatsuya Higaki;笠原一人;牧山康志;山盛弥生;山根聡;西木政統;笠原一人;石井剛;太田佳鈴;松村耕光;Mikio Nakahara;加藤尚武;平子友長;丸山士郎
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蘭渓道隆の頂相―建仁寺西来院調査報告を中心に
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  • DOI:
  • 发表时间:
    2014
  • 期刊:
  • 影响因子:
    0
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    ABE Hisashi ;WATANABE Ken;ISHIKAWA Atsuko;NOSHIRO Shuichi;FUJII Tomoyuki;IWASA Mitsuharu;KANEKO Hiroyuki;WADA Hiroshi;山盛弥生;宮下 修一;村田雄二郎;Tatsuya Higaki;笠原一人;牧山康志;山盛弥生;山根聡;西木政統;笠原一人;石井剛;太田佳鈴;松村耕光;Mikio Nakahara;加藤尚武;平子友長;丸山士郎;太田佳鈴;平子友長;TONAGA Yasushi;郡司ペギオ幸夫;浅見龍介
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Defects and charge compensation in wide bandgap semiconductors
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  • DOI:
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    0
  • 作者:
    OHASHI Naoki;SAKAGUCHI Isao;WATANABE Ken;ADACHI Yutaka;TAKESHI OGAKI;HISHITA Shunichi;MIYAZAKI Hiroki;Jesse Willia;ISHIGAKI Takamasa
  • 通讯作者:
    ISHIGAKI Takamasa
National Wealth without military strength?: The Four Postwar Eras in Modern East Asia
国家财富没有军事实力?
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    ABE Hisashi ;WATANABE Ken;ISHIKAWA Atsuko;NOSHIRO Shuichi;FUJII Tomoyuki;IWASA Mitsuharu;KANEKO Hiroyuki;WADA Hiroshi;山盛弥生;宮下 修一;村田雄二郎
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Non-destructive method for species identification of historical wooden artifacts using NIR spectroscopy: separation of softw
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  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    ABE Hisashi ;WATANABE Ken;ISHIKAWA Atsuko;NOSHIRO Shuichi;FUJII Tomoyuki;IWASA Mitsuharu;KANEKO Hiroyuki;WADA Hiroshi
  • 通讯作者:
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WATANABE Ken的其他文献

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{{ truncateString('WATANABE Ken', 18)}}的其他基金

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利用再生骨料减少环保型高流动性延性纤维混凝土的收缩,提高建筑物的耐久性
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  • 财政年份:
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    $ 2.46万
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    Grant-in-Aid for Scientific Research (C)
synergistic effects of functional constituents in food and antiviral drug: is it possible to overcome drug-resistance and to prevent flu?
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    16K00853
  • 财政年份:
    2016
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    $ 2.46万
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    Grant-in-Aid for Scientific Research (C)
Development of Environment-Friendly High-Fluidity Ductile-Fiber-Reinforced Concrete Using Recycled Aggregate for Seismic Performance Improvement of Reinforced Concrete Buildings
利用再生骨料开发环保型高流动性延性纤维混凝土以提高钢筋混凝土建筑的抗震性能
  • 批准号:
    15K06307
  • 财政年份:
    2015
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    $ 2.46万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Screening for antiviral compounds targeting nuclear export system from natural resources
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  • 批准号:
    25460125
  • 财政年份:
    2013
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    $ 2.46万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Demelopment of a realtime PCR-based semen identification method using the semen-specific hypomethylated DNA region
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    24790650
  • 财政年份:
    2012
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    $ 2.46万
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    Grant-in-Aid for Young Scientists (B)
Relationship between micro-cracks and time-dependent volume change in cementitious composite
水泥基复合材料中微裂纹与随时间变化的体积变化之间的关系
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    21760344
  • 财政年份:
    2009
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Functional compensation and independency of two sphingomyelin synthases
两种鞘磷脂合酶的功能补偿和独立性
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    2008
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Analysis of nuclear export of influenza viral RNP and search for novel inhibitors using the mechanisms
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  • 财政年份:
    2008
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