The role of type I interferon for the lower respiratory infection induced by Haemophilus influenzae

Ⅰ型干扰素对流感嗜血杆菌所致下呼吸道感染的作用

• 批准号: 24791026
• 负责人: NAKAMURA Shigeki
• 金额: $ 2.41万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791026/
• 关键词: 感染免疫; 自然免疫; インフルエンザ菌; I型インターフェロン; 細胞内侵入; 慢性下気道感染症

We examined the mechanism of chronic lower respiratory infection (cLRT) induced by Haemophilus influenzae with focusing the tissue-invasive ability especially bronchial epithelial cells. Twenty-one clinical isolates of H.influenzae were measured the ability of tissue-invasion and there were significant differences of the invasion ability between each strain. In addition, the production of type I IFN in the supernatant of the cell lines infected with high-invasive strains has increased significantly compared to the low-invasive strains.In general, type I IFN has the inhibitory effect against the viral infection, however we found new insight of type I IFN to accelerate the LRT infection induced with H.influenzae, and this finding will be contribute to elucidate the mechanism of bacterial cLRT.

本研究从流感嗜血杆菌对组织尤其是支气管上皮细胞的侵袭能力出发，探讨了流感嗜血杆菌引起慢性下呼吸道感染的机制。 对21株流感嗜血杆菌临床分离株进行了组织侵袭能力测定，各菌株的侵袭能力差异有统计学意义。 另外，高侵袭性菌株感染细胞后，细胞培养上清中I型干扰素的产生量较低侵袭性菌株显著增加，总体而言，I型干扰素对病毒感染具有抑制作用，但我们发现了I型干扰素加速流感嗜血杆菌诱导的LRT感染的新见解，这一发现将有助于阐明细菌cLRT的机制。