Development of the structure analytical method for protein having difficulty in crystallization by the solid-state NMR

开发难结晶蛋白质的固体核磁共振结构分析方法

• 批准号: 24750014
• 负责人: OHASHI Ryutaro
• 金额: $ 2.83万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24750014/
• 关键词: 分子構造; 固体ＮＭＲ; タンパク質; 原子核間距離; 立体構造解析

In this study, the distances between two carbon nuclei in amino acids were estimated from time-dependence of signals recorded by measurements of two-dimensional exchange solid-state nuclear magnetic resonance. We analyzed two amino acids (l-Leucine, l-Alanine) which were labeled two carbon nuclei with carbon-13. We succeeded to estimate the distance between the two carbons of methine and methyl groups in l-Alanine from 0.9 to 1.6 angstroms. Although it was estimated that the distance between carbon nuclei of methine and carboxyl groups in l-Leucine was less than 1.6 angstroms, we could not estimate the lower limit of the distance of l-Leucine. From the above, it is indicated that ranges of distances among carbon nuclei directly-bonding hydrogen nuclei are possibly estimated.

在这项研究中，通过二维交换固态核磁共振测量记录的信号的时间依赖关系来估计氨基酸中两个碳核之间的距离。我们分析了用~(13)C标记的两个碳核的两个氨基酸(L-亮氨酸、L-丙氨酸)。我们成功地估算了L丙氨酸中亚甲基和甲基的两个碳之间的距离为0.9埃到1.6埃。虽然估计了L-亮氨酸中甲基碳核与羧基之间的距离小于1.6埃，但不能估计L-亮氨酸的距离下限。由此可知，碳核与氢核直接键合的距离范围是可能的。