Temperature-modulated selective cell adhesion and detachment using intelligent bio-interface and application of them to cell separation

为了制备热调节的生物界面，在不修饰细胞表面的情况下分离细胞，用于再生医学和组织工程，在玻璃基板上形成热响应性疏水共聚物刷。通过观察四种人细胞在37℃下的粘附和在20℃或10℃下的脱落，对制备的共聚物刷状表面进行了表征。内皮细胞和成纤维细胞的有效分离温度分别为20℃和10℃。利用细胞固有的温度敏感性脱离共聚物刷，分离内皮细胞和成纤维细胞的混合物。

For preparing thermally modulated bio-interface that separates cells without the modification of cell surfaces for regenerative medicine and tissue engineering, thermo-responsive hydrophobic copolymer brushes were formed on glass substrate. Prepared copolymer brush surfaces were characterized by observing the adhesion at 37 C and detachment at 20 C or 10 C of four types of human cells. Endothelial cell and fibroblast exhibited their effective detachment temperature at 20 C and 10 C, respectively. Using cells' intrinsic temperature sensitivity for detachment from the copolymer brush, a mixture of endothelial cell and fibroblast was separated.