Analyses of nuclear envelope dynamics using a novel model fission yeast

使用新型裂殖酵母模型分析核膜动力学

• 批准号: 24770192
• 负责人: AOKI KEITA
• 金额: $ 3万
• 依托单位: National Institute of Genetics
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770192/
• 关键词: 核膜動態; 核膜崩壊; 分裂期制御; 細胞周期; S. japonicus; 国際情報交換; アメリカ

For the inheritance of genetical information, a nuclear membrane, surrounding DNA, has to be inherited to daughter cells correctly. Higher eukariotic cells like human have 'open' mitosis system accompanying breakdown and regeneration of nuclear membrane. On the other hand, lower eukariotic cells like yeasts have 'closed' mitosis system accompanying shrinkage of nuclear membrane. The significance and mechanism of the difference has not been understood so far.For our study, a fission yeast S. japonicus, which has 'semi-open' mitosis, was used. Searching genes relating to inheritance of nuclear membrane by genetical screening, it was found that APC/Cyclosome and fatty acid synthase function for the inheritance of nuclear membrane. Because APC/Cyclosome is a key factor in the protein degradation pathway, and fatty acids are important for constructing membrane, we proposed a model that regulation of level of nuclear membrane via protein degradation is necessary for the inheritance of DNA.

为了遗传遗传信息，围绕DNA的核膜必须正确地遗传给子代细胞。高等真核细胞与人类一样，具有开放的有丝分裂系统，伴随着核膜的破裂和再生。另一方面，低等真核细胞如酵母具有封闭的有丝分裂系统，并伴有核膜的收缩。到目前为止，这种差异的意义和机制还不清楚。在我们的研究中，使用了一种具有‘半开放’有丝分裂的分裂酵母。通过遗传筛选寻找与核膜遗传相关的基因，发现APC/环体和脂肪酸合成酶在核膜遗传中起作用。由于APC/环体是蛋白质降解途径中的关键因子，而脂肪酸是构建膜的重要因素，我们提出了一个通过蛋白质降解来调节核膜水平的模型，这是DNA遗传所必需的。

项目成果

Genome-wide identification and characterization of replication origins by deep sequencing.

• DOI: 10.1186/gb-2012-13-4-r27
• 发表时间: 2012-04-24
• 期刊: Genome biology
• 影响因子: 12.3
• 作者: Xu J;Yanagisawa Y;Tsankov AM;Hart C;Aoki K;Kommajosyula N;Steinmann KE;Bochicchio J;Russ C;Regev A;Rando OJ;Nusbaum C;Niki H;Milos P;Weng Z;Rhind N
• 通讯作者: Rhind N

染色体と核膜構造の維持に必要な因子の解析

维持染色体和核膜结构所需因素的分析

• 发表时间: 2013
• 作者: 青木敬太;志波優;高田啓;吉川博文;仁木宏典
• 通讯作者: 仁木宏典

Semi-open mitosisの進行に必須な核膜動態制御の解析、第30回染色体ワークショップ

半开放有丝分裂进展所必需的核膜动力学控制分析，第 30 届染色体研讨会

• 发表时间: 2012
• 作者: 青木敬太;志波優;高田啓;吉川博文;仁木宏典
• 通讯作者: 仁木宏典

仁木研の主要な研究テーマ

Niki Lab的主要研究主题

Regulation of nuclear envelope dynamics via APC/C is necessary for the progression of semi-open mitosis in Schizosaccharomyces japonicus

• DOI: 10.1111/gtc.12072
• 发表时间: 2013-09-01
• 期刊: GENES TO CELLS
• 影响因子: 2.1
• 作者: Aoki, Keita;Shiwa, Yuh;Niki, Hironori
• 通讯作者: Niki, Hironori