Analyses of nuclear envelope dynamics using a novel model fission yeast

为了遗传信息的遗传，必须将包围DNA的核膜正确地遗传给子细胞。高等真核细胞与人类一样，具有“开放”的有丝分裂系统，伴随着核膜的破裂和再生。另一方面，较低的真核细胞，如酵母，有“封闭”的有丝分裂系统，伴随着核膜的收缩。这种差异的意义和机制目前还不清楚。使用了具有“半开放”有丝分裂的细胞。通过遗传学筛选寻找与核膜遗传相关的基因，发现APC/Cyclosome和脂肪酸合成酶在核膜遗传中起作用。由于APC/Cyclosome是蛋白质降解途径中的关键因子，而脂肪酸对于构建膜是重要的，因此我们提出了通过蛋白质降解调节核膜水平是DNA遗传所必需的模型。

For the inheritance of genetical information, a nuclear membrane, surrounding DNA, has to be inherited to daughter cells correctly. Higher eukariotic cells like human have 'open' mitosis system accompanying breakdown and regeneration of nuclear membrane. On the other hand, lower eukariotic cells like yeasts have 'closed' mitosis system accompanying shrinkage of nuclear membrane. The significance and mechanism of the difference has not been understood so far.For our study, a fission yeast S. japonicus, which has 'semi-open' mitosis, was used. Searching genes relating to inheritance of nuclear membrane by genetical screening, it was found that APC/Cyclosome and fatty acid synthase function for the inheritance of nuclear membrane. Because APC/Cyclosome is a key factor in the protein degradation pathway, and fatty acids are important for constructing membrane, we proposed a model that regulation of level of nuclear membrane via protein degradation is necessary for the inheritance of DNA.