Function of homeodomain transcription factor pKnox1 on the maintain and differentiation of spermatogonia

同源域转录因子pKnox1对精原细胞维持和分化的作用

• 批准号: 24780290
• 负责人: KAWAI yasuhiro
• 金额: $ 2.91万
• 依托单位: National Institute of Infectious Diseases (2013)Tokyo University of Science (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24780290/
• 关键词: pKnox1; 精原細胞; 増殖

The maintenance, self-renewal, and differentiation of spermatogonial stem cells (SSCs) are tightly regulated processes that are critical for normal spermatogenesis. TALE family homeodomain transcription factors, including pKnox1, are demonstrated to be essential for hematopoietic stem cell maintenance and self-renewal. pKnox1 is preferentially expressed in the male germ cell lineage, there is a possibility that pKnox1 is involved in SSC maintenance and differentiation. Our study shown that pKnox1 deleted spermatogonia was arrested at c-kit positive spermatogonia and proliferative capacity of c-kit positive spermatogonia was significantly reduced. Moreover, at 1 week after pKnox1 deletion induced by tamoxifen treatment, spermatogenesis was normal, although proliferation related genes expression pattern were dynamically changed compared with control testes. These results indicated that pKnox1 is required for proper proliferation of c-kit positive spermatogonia.

精原干细胞（spermatogonialstemcells，SSCs）的维持、自我更新和分化是一个受到严格调控的过程，对正常的精子发生至关重要。TALE家族同源结构域转录因子，包括pKnox 1，被证明是造血干细胞维持和自我更新所必需的。pKnox 1在雄性生殖细胞系中优先表达，pKnox 1可能参与SSC的维持和分化。我们的研究表明，pKnox 1缺失的精原细胞在c-kit阳性精原细胞处被阻滞，c-kit阳性精原细胞的增殖能力显著降低。此外，在1周后，pKnox 1删除诱导他莫昔芬治疗，精子发生是正常的，虽然增殖相关基因的表达模式与对照组相比，动态变化。这些结果表明pKnox 1是c-kit阳性精原细胞正常增殖所必需的。

项目成果

Tlx1-CreER-Venus Mice

Tlx1-CreER-维纳斯小鼠

• 发表时间: 2014
• 期刊: A New Tool for the Study of Spleen Development
• 作者: Ryo Nakahara;Yasuhiro Kawai;Akihisa Oda;Akikazu Murakami;Takachika Azuma;Yoichiro Iwakura;and Ryo Goitsuka
• 通讯作者: and Ryo Goitsuka

Homeodomain transcription factor pKnox1 is required for proliferation of c-kit-positive spermatogonia

c-kit 阳性精原细胞增殖需要同源域转录因子 pKnox1

• 发表时间: 2012
• 作者: kawai yasuhiro;Goitsuka ryo
• 通讯作者: Goitsuka ryo

ホメオドメイン転写因子pKnox1はc-kit陽性精原細胞の増殖および生存性を正に制御する

同源域转录因子 pKnox1 正向调节 C-kit 阳性精原细胞的增殖和活力

• 发表时间: 2012
• 作者: Ryo Nakahara;Yasuhiro Kawai;Akihisa Oda;Akikazu Murakami;Takachika Azuma;Yoichiro Iwakura;and Ryo Goitsuka;Yasuhiro Kawai,Ryo Goitsuka
• 通讯作者: Yasuhiro Kawai,Ryo Goitsuka