Development of prodrug-siRNA activated by intracellular reducing environment

点击翻译按钮获取中文摘要

In this study, we developed 2'-O-methyldithiomethyl (MDTM)-RNA as a prodrug-RNA. The MDTM-RNAs showed high stability under fetal bovine serum, and were easily converted into native-RNAs under glutathione reducing condition found in the intracellular environment. In addition, the MDTM-RNAs showed enough affinity toward complementary RNA. The synthesis of MDTM-RNAs was effectively achieved by a post-synthetic approach. The MDTM-RNA might be an excellent candidate for RNA technologies such as RNA interference.