New Transgenic Mouse System to Analyze Functional Mechanisms of Transcription Factors

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Transcription factors form multi-protein complexes with various partner proteins including non-DNA binding co-factors and chromatin remodeling enzymes, and regulate expression patterns of numerous target genes. In this study, we generated transgenic mouse lines that express a transcription factor containing Tandem Affinity Purification (TAP)-tag. These transgenic mice express TAP-tag proteins tissue-dependently in an inducible manner, and are applicable to various experiments such as mass spec analyses of transcriptional co-factors as well as ChIP-sequening of target genes. Such studies will help dissection of molecular mechanisms of in vivo transcriptional regulation.