Functional analysis of a signal transduction system in pathogenic fungi Aspergillus fumigatus

病原真菌烟曲霉信号转导系统的功能分析

• 批准号: 24790410
• 负责人: HAGIWARA Daisuke
• 金额: $ 2.33万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790410/
• 关键词: 情報伝達系; 二成分制御系; ヒスチジンキナーゼ; MAPKカスケード; 浸透圧ストレス; 環境応答機構; 情報伝達機構; MAPK

Recently, fungal infection has increased. In order to understand how the causative fungus Aspergillus fumigatus survives in a stressful environment and during infection, we focused on a stress response signaling mechanism, High Osmolar Glycerol (HOG) pathway. We constructed the gene deletion mutants of each component of the pathway, and investigated the components. We found that one histidine kinase, NikA, is involved in conidiation, hyphal morphogenesis, osmotic stress resistance, and fungicide response in A. fumigatus. These results will be useful for further understanding of the response mechanism against extracellular stresses and also for development of new drugs targeting this signaling pathway in A. fumigatus.

最近，真菌感染增加了。为了了解烟曲霉的因果真菌如何在压力环境中和感染期间生存，我们专注于应力响应信号传导机制高渗透甘油（HOG）途径。我们构建了途径每个成分的基因缺失突变体，并研究了组件。我们发现一种组氨酸激酶Nika参与了烟曲霉中的分生物，菌丝形态发生，渗透胁迫抗性和杀菌剂反应。这些结果将有助于进一步理解针对细胞外应激的反应机制，以及针对烟曲霉中这种信号通路的新药物的开发。

项目成果

Aspergillus fumigatusの多様なストレスおよび薬剤に対する浸透圧応答経路の機能解析

烟曲霉对各种胁迫和药物的渗透响应途径的功能分析

• 发表时间: 2012
• 作者: 萩原大祐;豊留孝仁;高橋梓;亀井克彦;五ノ井透;川本進
• 通讯作者: 川本進

The effects of cultivation temperature on characteristics of conidia in pathogenic fungus Aspergillus fumigatus

培养温度对病原真菌烟曲霉分生孢子特性的影响

• 发表时间: 2014
• 作者: Hagiwara D;Suzuki S;Kamei K;Gonoi T;Kawamoto S
• 通讯作者: Kawamoto S

カビ胞子のストレス耐性機構の解明をめざして

旨在阐明霉菌孢子的抗逆机制

• 发表时间: 2013
• 作者: 萩原大祐;五ノ井透;川本進;萩原大祐
• 通讯作者: 萩原大祐

Repression of the phosphor-transmitter gene ypdA resulting in growth defect in Aspergillus fumigatus

磷递质基因 ypdA 的抑制导致烟曲霉生长缺陷

• 发表时间: 2013
• 作者: Hagiwara D;Takahashi H;Nakayama M;Abe K;Gonoi T;Kawamoto S.
• 通讯作者: Kawamoto S.

A novel transcriptional regulator AtrR of Aspergillus fumigatus is required for azole resistance, hypoxia growth, and expression of ABC transporter gene cdr1B

烟曲霉的新型转录调节因子 AtrR 是唑类抗性、缺氧生长和 ABC 转运蛋白基因 cdr1B 表达所必需的

• 发表时间: 2014
• 作者: Hagiwara D;Shimizu K;Ohba A;Kamei K;Gonoi T;Kawamoto S
• 通讯作者: Kawamoto S