Impulsive strain-induced axonal injury and functional analysis of cell-to-cell communication in neurons

脉冲应变诱导的轴突损伤和神经元细胞间通讯的功能分析

• 批准号: 24700459
• 负责人: NAKADATE Hiromichi
• 金额: $ 2.91万
• 依托单位: Tokyo Metropolitan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24700459/
• 关键词: 頭部外傷; びまん性軸索損傷; アミロイド前駆体タンパク質; 脳神経細胞; 細胞引張実験; 衝撃ひずみ; 免疫蛍光染色; 微細加工; 軸索損傷; 軸索伸長方向制御; 神経活動電位; β-アミロイド前駆体タンパク質

Traumatic brain injury caused by traffic accidents, blow, falls and contact sports, is an important public health problem throughout societies because of a contribution of disability and death. While diffuse axonal injury (DAI) is one of the most serious traumatic brain injuries, DAI is caused by sudden inertial loading to the head associated with rapid deformation of brain tissue, resulting in the stretching of neural axons. In this study, the cultured rat brain neuronal cells were stretched, the beta-amyloid precursor protein (beta-APP) that is conveyed by axonal transport accumulates where axonal transport is disrupted, was stained and observed using fluorescence microscopy.The results show that the threshold of interruption of axonal transport is strains of 15-22% at strain rates of 21-27/s and the accumulation of beta-APP is a quantitative marker for traumatic axonal injury at a cellular level.

交通事故、撞击、跌倒和接触性运动造成的创伤性脑损伤是整个社会的一个重要公共卫生问题，因为它造成了残疾和死亡。弥漫性轴索损伤(DAI)是最严重的创伤性脑损伤之一，DAI是由于脑组织迅速变形，导致神经轴突伸展而导致头部突然惯性负荷所致。本研究对培养的大鼠脑神经元细胞进行拉伸，对轴突运输中断处的β-淀粉样前体蛋白(β-APP)进行染色和荧光显微镜观察。结果表明，在21-27/S的应变率下，轴突运输中断的阈值为15-22%，β-APP的积累是细胞水平上创伤性轴突损伤的定量标志。

项目成果

伸長方向制御した脳神経軸索の引張損傷評価

受控伸长方向的脑神经轴突拉伸损伤评估

• 发表时间: 2014
• 作者: Nakadate;H.;Aomura;S. and Kakuta;A.;久保田麻紀,田地川勉,池本敏行,田窪孝行,大場謙吉;鶴見明冴美,中楯浩康,青村茂,角田陽
• 通讯作者: 鶴見明冴美,中楯浩康,青村茂,角田陽

マイクロフルイディクスを用いた神経軸索伸長制御の細胞引張試験への応用

微流体控制神经轴突生长在细胞张力测试中的应用

• 发表时间: 2013
• 作者: Nakadate;H.;Aomura;S. and Kakuta;A.;菊田和紘,中楯浩康,角田陽,青村茂;加藤陽介,郡慎平,田地川勉,板東潔,山本恭史;能田卓弥,久保田麻紀,田地川勉,池本敏行,田窪孝行;中楯浩康,金子由磨,菊田和紘,青村茂,角田陽;加藤陽介,郡慎平,田地川勉,板東潔;中楯浩康
• 通讯作者: 中楯浩康

Evaluation of Stretch-injured Axon in Brain Neuronal Cell by Observation of β-Amyloid Precursor Protein

通过观察 β-淀粉样前体蛋白评估脑神经细胞拉伸损伤的轴突

• 发表时间: 2014
• 作者: Nakadate;H.;Kaneko;Y.;Kikuta;K.;Aomura;S. and Kakuta;A.
• 通讯作者: A.

An in Vitro Stretch-Injury Model for Elongation-Controlled Neuronal Cells: Effect of Strains Along Neurite

伸长控制神经元细胞的体外拉伸损伤模型：沿神经突的应变效应

• DOI: 10.1007/978-3-319-02913-9_194
• 发表时间: 2014
• 期刊: International Federation for Medical & Biological Engineering proceedings
• 作者: Nakadate;H.;Aomura;S. and Kakuta;A.
• 通讯作者: A.

Damage Evaluation in Cultured Neuron Exposed to Two Dimensional Tensile Stress

暴露于二维拉伸应力的培养神经元的损伤评估

• DOI: 10.1299/kikaia.78.1090
• 发表时间: 2012
• 期刊: TRANSACTIONS OF THE JAPAN SOCIETY OF MECHANICAL ENGINEERS Series A
• 作者: 中楯 浩康;馬橋 洋人;張 月琳;角田 陽;青村 茂
• 通讯作者: 青村 茂