Impulsive strain-induced axonal injury and functional analysis of cell-to-cell communication in neurons

交通事故、撞击、跌倒和接触性运动造成的创伤性脑损伤是整个社会的一个重要公共卫生问题，因为它造成了残疾和死亡。弥漫性轴索损伤(DAI)是最严重的创伤性脑损伤之一，DAI是由于脑组织迅速变形，导致神经轴突伸展而导致头部突然惯性负荷所致。本研究对培养的大鼠脑神经元细胞进行拉伸，对轴突运输中断处的β-淀粉样前体蛋白(β-APP)进行染色和荧光显微镜观察。结果表明，在21-27/S的应变率下，轴突运输中断的阈值为15-22%，β-APP的积累是细胞水平上创伤性轴突损伤的定量标志。

Traumatic brain injury caused by traffic accidents, blow, falls and contact sports, is an important public health problem throughout societies because of a contribution of disability and death. While diffuse axonal injury (DAI) is one of the most serious traumatic brain injuries, DAI is caused by sudden inertial loading to the head associated with rapid deformation of brain tissue, resulting in the stretching of neural axons. In this study, the cultured rat brain neuronal cells were stretched, the beta-amyloid precursor protein (beta-APP) that is conveyed by axonal transport accumulates where axonal transport is disrupted, was stained and observed using fluorescence microscopy.The results show that the threshold of interruption of axonal transport is strains of 15-22% at strain rates of 21-27/s and the accumulation of beta-APP is a quantitative marker for traumatic axonal injury at a cellular level.