Prevention of photoaging by scavenging mitochondrial singlet oxygen

通过清除线粒体单线态氧预防光老化

• 批准号: 24700762
• 负责人: WOLF Alexander
• 金额: $ 2.83万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24700762/
• 关键词: 光老化; 老化; 酸化ストレス; 皮膚; skin; oxidative stress; aging; GFP

Due to a high desire to look young and beautiful, cosmetics to slow down aging are a large market. Wrinkle formation has been attributed to UVA radiation.Using hairless transgenic mice expressing a redox-sensitive GFP (roGFP1) in the cytosol and in mitochondria of epidermal keratinocytese, we discovered that UVA induces oxidative stress in skin mitochondria but not cytosol. We used in vivo oxidative stress imaging to evaluate singlet oxygen scavengers. Topical application of carotenoids did not prevent UVA-induced oxidative stress. We assume this was due to poor permeation of the large carotenoids across the stratum corneum. However, a low molecular weight compound able to permeate the stratum corneum well could reduce the amount of oxidation induced by UVA relative to vehicle control.Future research will further investigate substances that can prevent UVA or visible light-induced oxidative stress by topical application to skin.

由于人们渴望看起来年轻美丽，延缓衰老的化妆品是一个很大的市场。皱纹的形成已归因于UVA radiation.Using无毛转基因小鼠表达的氧化还原敏感的绿色荧光蛋白（roGFP1）的细胞质和表皮角质形成细胞的线粒体，我们发现，UVA诱导皮肤线粒体的氧化应激，而不是细胞质。我们使用体内氧化应激成像来评估单线态氧清除剂。局部应用类胡萝卜素不能防止UVA诱导的氧化应激。我们认为这是由于大类胡萝卜素穿过角质层的渗透性差。然而，低分子量的化合物能够很好地渗透角质层，可以减少由UVA诱导的氧化量相对于媒介物control.Future研究将进一步调查的物质，可以防止UVA或可见光诱导的氧化应激通过局部应用于皮肤。

项目成果

Real-time monitoring of oxidative stress in live mouse skin.

• DOI: 10.1038/jid.2013.428
• 发表时间: 2014-06
• 期刊: The Journal of investigative dermatology
• 作者: Alexander M. Wolf;K. Nishimaki;Naomi Kamimura;S. Ohta

Oxidative stress in hairless mouse epidermis in response to visible and ultraviolet light

无毛小鼠表皮对可见光和紫外线的氧化应激反应

• 发表时间: 2014
• 作者: Rikimaru N;Ohsawa Y;Wolf AM;Nishimaki K;Ichimiya H;Kamimura N;Nishimatsu S;Ohta S;Sunada Y;Wolf Alexander
• 通讯作者: Wolf Alexander

Taurine Ameliorates Impaired the Mitochondrial Function and Prevents Stroke-like Episodes in Patients with MELAS

• DOI: 10.2169/internalmedicine.51.7529
• 发表时间: 2012-01-01
• 期刊: INTERNAL MEDICINE
• 影响因子: 1.2
• 作者: Rikimaru, Mitsue;Ohsawa, Yutaka;Sunada, Yoshihide
• 通讯作者: Sunada, Yoshihide

紫外線による皮膚の生体内ストレスイメージング

使用紫外线对皮肤进行体内应力成像

• 发表时间: 2013
• 作者: Wolf Alexander;中島裕也;上村尚美;西槙貴代美;太田成男
• 通讯作者: 太田成男

日本医科大学老人病研究所（細胞生物学分野）

日本医科大学老年医学研究所（细胞生物学系）