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Light stress proteins from the chlorophyll a/b-binding gene family in Arabidopsis thaliana

拟南芥叶绿素 a/b 结合基因家族的光应激蛋白

基本信息

批准号：
5423272
负责人：
Professorin Dr. Iwona Adamska
金额：
--
依托单位：
Lehrstuhl für Physiologie und Biochemie der Pflanzen
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2004
资助国家：
德国
起止时间：
2003-12-31 至 2011-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/5423272?language=en
关键词：
Light stress proteins chlorophyll binding

项目摘要

The family of light-harvesting chlorophyll a/b-binding proteins in higher plants and green algae is composed of more than 20 different members associated with photosystem I and II. In addition, several distant relatives of this family with conserved chlorophyll-binding residues and a transient expression pattern related to various stress conditions have been reported. These distant relatives include: three-helix Elips (early light-induced proteins) and related proteins, two helix Seps (stress-enhanced proteins) and one-helix Ohps (one-helix proteins). While Elips are not detected under ambient light conditions, a significant amount of Seps and Ohps is present in the absence of light stress but their level increases drastically after exposure of leaves to high intensity light. In the past years our interest was focused on two such proteins, Sep2 and Ohp2. We would like to continue studies on location and function(s) of these proteins in Arabidopsis thaliana and broaden our analysis by including two additional members, Sep3 and Ohp1. We plan the overexpression and raising polyclonal antibodies for Sep3 and Ohp1 and reconstitution of overexpressed proteins with isolated pigments. Localization studies will be performed by subfractionation of thylakoid membranes and protein-protein interaction will be analyzed using crosslinking or a surface plasmon resonance technology (Biacore). Reverse genetics (analysis "knock out", "knock down" mutants and transgenics) will be applied to unravel the function of these proteins in the chloroplast.

高等植物和绿藻的光捕获叶绿素a/b结合蛋白家族由与光系统I和II相关的20多个不同成员组成。此外，还报道了该家族的几个远亲，它们具有保守的叶绿素结合残基和与各种胁迫条件相关的瞬时表达模式。这些远亲包括：三螺旋elps（早期光诱导蛋白）和相关蛋白，两螺旋Seps（应力增强蛋白）和一螺旋Ohps（单螺旋蛋白）。虽然在环境光条件下无法检测到elps，但在没有光胁迫的情况下存在大量的Seps和ops，但在叶片暴露于高强度光后，它们的水平急剧增加。在过去的几年里，我们的兴趣集中在两种这样的蛋白质上，Sep2和op2。我们希望继续研究这些蛋白在拟南芥中的位置和功能，并通过加入另外两个成员Sep3和op1来扩大我们的分析。我们计划过表达和提高Sep3和Ohp1的多克隆抗体，并利用分离的色素重构过表达蛋白。定位研究将通过类囊体膜的亚分进行，蛋白质-蛋白质相互作用将使用交联或表面等离子体共振技术（Biacore）进行分析。反向遗传学（分析“敲除”、“敲除”突变体和转基因）将用于揭示这些蛋白质在叶绿体中的功能。