The Structure and Function of Reggie/Flotillin-like Proteins in Arabidopsis thaliana

拟南芥Reggie/Flotillin样蛋白的结构和功能

• 批准号: 22177751
• 负责人: Professorin Dr. Iwona Adamska
• 金额: --
• 依托单位: Arbeitsgruppe Neurobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2006-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/22177751?language=en
• 关键词: Structure; Function; Reggie; Flotillin; like

Reggies, discovered in C. Stuermer lab, are ubiquitous plasma membrane-associated intracellular proteins and characteristic markers of lipid raft microdomains. Metazoan Reggies have been implicated in axon regeneration, cell process and contact formation and in providing functional platforms for cell signaling. Although Reggie-like proteins are present in distant phyla, such as bacteria, fungi and higher plants, little is known about their physiological role in these organisms. We found three reggie-like genes (called here re-l1a, -l1b and -l2) in the genome of Arabidopsis thaliana and investigated their evolution, expression and function. We demonstrated that transcripts for Re-l1a and –l1b are upregulated during early stages of seedling development and later on during senescence as well as under light stress conditions. We succeeded to isolate a “knock-out” homozygous mutant line for Re-l2 that shows delayed development. The deletion of re-l1a gene seemed to be lethal. Here we propose to investigate the function and structural characteristics of Reggie-like proteins in plants, in order to compare them with their vertebrate and bacterial counterparts because of striking similarities of protein domain composition and lipid raft association. We will further examine the expression of Reggie-like transcripts and proteins under different physiological conditions, in various plant organs and tissues, to reveal their subcellular location laying emphasis on lipid raft association, search for interacting partners, and perform functional analysis using reverse genetics. An attempt will be made to solve the 3D structure of plant Reggie-like proteins.

由C.Stuermer实验室发现的REGES是普遍存在的质膜相关细胞内蛋白，也是脂筏微域的特征标记。后生动物调节因子参与轴突再生、细胞加工和接触形成，并为细胞信号转导提供功能平台。虽然类Reggie蛋白存在于细菌、真菌和高等植物等遥远的门中，但人们对它们在这些生物体中的生理作用知之甚少。我们在拟南芥基因组中发现了三个类Reggie基因(这里称为Re-L1a、-L1B和-L2)，并对它们的进化、表达和功能进行了研究。我们证明了Re-L1a和-L1B的转录在幼苗发育的早期、后期的衰老和轻度胁迫条件下上调。我们成功地分离到了一株发育迟缓的Re-L2“敲除”纯合子突变株。Re-L1a基因的缺失似乎是致命的。在这里，我们打算研究植物中类Reggie蛋白的功能和结构特征，以便与脊椎动物和细菌的同类蛋白进行比较，因为它们在蛋白结构域组成和脂筏结合方面具有惊人的相似性。我们将进一步研究在不同生理条件下，不同植物器官和组织中Reggie样转录本和蛋白质的表达，以揭示它们的亚细胞定位，重点是脂筏结合，寻找相互作用的伙伴，并利用反向遗传学进行功能分析。将尝试解决植物类Reggie蛋白的3D结构。