抗アテローム性動脈硬化症治療薬評価のための三次元微小血管チップ

三维微血管芯片用于抗动脉粥样硬化药物评价

• 批准号: 14F04720
• 负责人: 松永 行子
• 金额: $ 1.47万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2014
• 资助国家: 日本
• 起止时间: 2014-04-25 至 2016-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14F04720/
• 关键词: 3D artery tissues; smooth muscle cells; endothelial cells; co-culture; collagen microchannel; inflammation; monocytes; lipids; vascular tissue

AIMS: This two-year JSPS postdoctoral fellowship (FY2014-2016) aims at fabricating a three-dimensional (3D) co-culture artery-on-a-chip to closely simulate the human vascular morphology, both in the healthy and inflammatory disease conditions. The longer-term goal of this project is to develop viable in vitro tools to support high-throughput therapeutic and biopharmaceutical research of various drugs and pharmaceutical formulated compounds.PROGRESS: To date, we have established two simple needle-based fabrication procedures to achieve controllable arterial architectures on a microchip; (a) an arteriole-like bilayer of human vascular smooth muscle cells (SMC) and endothelial cells (EC), and (b) an artery-like structure composed of multilayers of SMC covered by an inner EC monolayer lining. These proof-of-concept microvascular models have been extensively validated for their robustness and reproducibility, as well as for physical stability and metabolic behaviors to mimic the healthy state of a blood vessel. Meanwhile, experiments are ongoing to incorporate various pathological factors into these artery-like tissues to induce vascular inflammatory disease.OUTCOME; The results obtained have formed the basis for a manuscript, which is near to submission (by April 2016) as a full length paper to an international tissue engineering journal. It also contributes to a poster presentation at the University of Tokyo Life Science Symposium (第16回東大生命科学シンポジウム) on April 23, 2016.

AIMS: This two-year JSPS postdoctoral fellowship (FY2014-2016) aims at fabricating a three-dimensional (3D) co-culture artery-on-a-chip to closely simulate the human vascular morphology, both in the healthy and inflammatory disease conditions. The longer-term goal of this project is to develop viable in vitro tools to support high-throughput therapeutic and biopharmaceutical research of various drugs and pharmaceutical formulated compounds.PROGRESS: To date, we have established two simple needle-based fabrication procedures to achieve controllable arterial architectures on a microchip; (a) an arteriole-like bilayer of human vascular smooth muscle cells (SMC) and endothelial cells (EC), and (b) an artery-like structure composed of multilayers of SMC covered by an inner EC monolayer lining. These proof-of-concept microvascular models have been extensively validated for their robustness and reproducibility, as well as for physical stability and metabolic behaviors to mimic the healthy state of a blood vessel. Meanwhile, experiments are ongoing to incorporate various pathological factors into these artery-like tissues to induce vascular inflammatory disease.OUTCOME; The results obtained have formed the basis for a manuscript, which is near to submission (by April 2016) as a full length paper to an international tissue engineering journal. It also contributes to a poster presentation at the University of Tokyo Life Science Symposium (the 16th Dongda Life Science Symposium) on April 23, 2016.

项目成果

A minimalist design of biomimetic microvascular models

仿生微血管模型的简约设计

• 发表时间: 2016
• 作者: Tan;A.;Matsunaga;Y.T.
• 通讯作者: Y.T.

Biomimetic microvascular chip for high throughput therapeutic research

用于高通量治疗研究的仿生微血管芯片

• 发表时间: 2014
• 作者: Tan;A.;Yukawa;Y.;Fujisawa;K.;Matsunaga;Y.T
• 通讯作者: Y.T