ベクターコントロールによる有機塩素系農薬汚染が家畜・ヒトに及ぼす毒性影響の解明

已经出版了两份手稿。在野外暴露于滴滴涕的鸡中，检测到了各种mRNA表达的变化，包括几种禽类β-防御素的下调。关于这些发现的手稿目前正处于修订阶段。体外暴露p，p‘-DDT后，使用qRT-PCR评估了mCF7人乳腺癌细胞中的mRNA表达。与氧化应激标志物、异源代谢酶和转运蛋白相关的几个基因在暴露后显著上调。已经提交了一份手稿供同行审查。对活体暴露于p，p‘-DDT的鸡的样本进行了进一步的分析。肝组织病理学检查未见明显改变。肝脏样本的QRT-PCR证实了禽β防御素(AvBD)基因AvBD1和AvBD7的基因表达改变。此外，使用亲水相互作用液相色谱(HILIC)的代谢组学分析显示，暴露组中许多与氨基酸代谢相关的代谢物显著升高。这些结果目前正在编写，以供期刊提交。

Two manuscripts have been published. Various alterations in mRNA expression were detected in chickens exposed to DDTs in the field, including down-regulation of several avian beta-defensins. A manuscript on these findings is currently at the revision stage of publication.mRNA expression was assessed using qRT-PCR after in vitro exposure of p,p’-DDT in MCF-7 human breast cancer cells. Several genes relating to oxidative stress markers, xenobiotic metabolizing enzymes and transporters were significantly up-regulated after exposure. A manuscript has been submitted for peer-review.Samples from in vivo exposure of chickens to p,p’-DDT underwent further analysis. Liver histopathology did not show significant changes. qRT-PCR of liver samples confirmed altered gene expression in avian beta defensin (AvBD) genes, AvBD1 and AvBD7. Further, metabolomics analysis using hydrophilic interaction liquid chromatography (HILIC) revealed many significantly elevated metabolites relating to amino acid metabolism in exposed groups. These results are currently being prepared for journal submission.