ベクターコントロールによる有機塩素系農薬汚染が家畜・ヒトに及ぼす毒性影響の解明

已出版两部手稿。在暴露于滴滴涕的鸡在外地，包括下调几个鸟类β-防御素的mRNA表达的各种变化。这些发现的手稿目前正处于出版的修订阶段。在MCF-7人乳腺癌细胞中体外暴露于p，p '-DDT后，使用qRT-PCR评估mRNA表达。与氧化应激标志物、异生物质代谢酶和转运蛋白相关的几个基因在暴露后显著上调。已提交了一份手稿供同行审查，并对鸡体内接触p，p '-滴滴涕的样本进行了进一步分析。肝组织病理学未见明显变化。肝脏样品的qRT-PCR证实了禽β防御素（AvBD）基因AvBD 1和AvBD 7的基因表达改变。此外，使用亲水相互作用液相色谱法（HILIC）进行的代谢组学分析显示，暴露组中与氨基酸代谢相关的许多代谢物显著升高。目前正在准备这些结果，以便提交期刊。

Two manuscripts have been published. Various alterations in mRNA expression were detected in chickens exposed to DDTs in the field, including down-regulation of several avian beta-defensins. A manuscript on these findings is currently at the revision stage of publication.mRNA expression was assessed using qRT-PCR after in vitro exposure of p,p’-DDT in MCF-7 human breast cancer cells. Several genes relating to oxidative stress markers, xenobiotic metabolizing enzymes and transporters were significantly up-regulated after exposure. A manuscript has been submitted for peer-review.Samples from in vivo exposure of chickens to p,p’-DDT underwent further analysis. Liver histopathology did not show significant changes. qRT-PCR of liver samples confirmed altered gene expression in avian beta defensin (AvBD) genes, AvBD1 and AvBD7. Further, metabolomics analysis using hydrophilic interaction liquid chromatography (HILIC) revealed many significantly elevated metabolites relating to amino acid metabolism in exposed groups. These results are currently being prepared for journal submission.