ベクターコントロールによる有機塩素系農薬汚染が家畜・ヒトに及ぼす毒性影響の解明

两篇手稿已经出版。在野外暴露于DDTs的鸡中检测到mRNA表达的各种变化，包括几种禽β -防御素的下调。关于这些发现的手稿目前正处于出版的修订阶段。在MCF-7人乳腺癌细胞中体外暴露p，p ' -DDT后，采用qRT-PCR技术评估mRNA表达。暴露后，与氧化应激标志物、外源代谢酶和转运蛋白相关的几个基因显著上调。一份手稿已提交同行评议。对鸡体内接触p，p ' -滴滴涕的样本进行了进一步分析。肝组织病理学未见明显变化。肝脏样本的qRT-PCR证实了AvBD基因、AvBD1和AvBD7基因表达的改变。此外，使用亲水相互作用液相色谱（HILIC）的代谢组学分析显示，暴露组中许多与氨基酸代谢相关的代谢物显著升高。这些结果目前正在准备提交给期刊。

Two manuscripts have been published. Various alterations in mRNA expression were detected in chickens exposed to DDTs in the field, including down-regulation of several avian beta-defensins. A manuscript on these findings is currently at the revision stage of publication.mRNA expression was assessed using qRT-PCR after in vitro exposure of p,p’-DDT in MCF-7 human breast cancer cells. Several genes relating to oxidative stress markers, xenobiotic metabolizing enzymes and transporters were significantly up-regulated after exposure. A manuscript has been submitted for peer-review.Samples from in vivo exposure of chickens to p,p’-DDT underwent further analysis. Liver histopathology did not show significant changes. qRT-PCR of liver samples confirmed altered gene expression in avian beta defensin (AvBD) genes, AvBD1 and AvBD7. Further, metabolomics analysis using hydrophilic interaction liquid chromatography (HILIC) revealed many significantly elevated metabolites relating to amino acid metabolism in exposed groups. These results are currently being prepared for journal submission.