Spectral Flow Cytometer

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An essential part of the research in the Department of General, Visceral and Transplant Surgery is the characterization and purification of cell populations from blood samples, dissociated tissue material, primary cell cultures or immortalized cell lines on the basis of their surface proteins in the murine, porcine or human system. For the research of the working group, a flow cytometer with a maximum of 16 parameters to be measured was available in Berlin. The use of the flow cytometer by the applicant's working group was constantly at a high level (approx. 650 - 1000 hours/year). The FACS device available at the MHH, in the research laboratory of the Clinic for General, Visceral and Transplant Surgery, can only measure 6 different parameters (4 colors + SSC, FSC). A new multispectral flow cytometer is indispensable for the continuation of the research activities. The requested multispectral flow cytometer is an instrument that measures the entire emitted fluorescence spectrum for each fluorochrome used, rather than individual spectral regions. This allows the use of fluorochromes with closely adjacent emission spectra. A multispectral flow cytometer equipped with five lasers can measure more than 40 parameters in parallel, allowing the analysis of complex cell phenotypes. In addition, signal quality and sensitivity can be improved by subtracting autofluorescence based on cell population. This is especially important for tissue-specific autofluorescence, but also for granulocytes or fluorescent proteins. A multispectral characterization of the cells under investigation allows a significant reduction in the number of samples required and thus in the amount of material and time needed. In view of the often very limited samples available, e.g. from murine animal models or patient biopsies, such an instrument allows a maximum gain of information. Populations within a cell mixture can be characterized in detail. Patient samples with a limited number of cells can be defined with high resolution and animal experiments can be reduced according to the 3R principles. We are requesting a multispectral flow cytometer that will meet the needs of cutting-edge research in the coming years. The requested instrument will allow us to continue the research of the past years, to expand existing research approaches and to enable new research projects.